HspA6 Overexpression Lysate (Native)


Western Blot: HspA6 Overexpression Lysate (Adult Normal) [NBL1-11754] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HspA6.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

HspA6 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207795 and based on accession number NM_002155. The protein contains a C-terminal DDK tag.
Homo sapiens heat shock 70kDa protein 6 (HSP70B') (HSPA6), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
70.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HspA6 Overexpression Lysate (Native)

  • heat shock 70 kDa protein 6
  • Heat shock 70 kDa protein B'
  • heat shock 70kD protein 6 (HSP70B')
  • heat shock 70kDa protein 6 (HSP70B')
  • HSP70B'


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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FAQs for HspA6 Lysate (NBL1-11754). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in the following references: NBL1-11754, NB820-59468, NBL1-14127,NBL1-07345. Have these references got any tag in their sequence for their purification, so the weight (KDa) increases?
    • NBL1-11754, NBL1-14127 and NBL1-07345 are all overexpression lysates; in each case, the protein contains a C-terminal Myc/DDK epitope tag (only 3 kDa total). The NB820-59468 is not an overexpression lysate; it is a whole normal brain (fetal) tissue lysate.

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Gene Symbol HSPA6