HspA6 Antibody (OTI1H3) - Azide and BSA Free

Western Blot: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HspA6.

Immunocytochemistry/Immunofluorescence: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HspA6.

Flow Cytometry: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HspA6 antibody, and then analyzed by flow cytometry.

Western Blot: HspA6 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70959] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HspA6 monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HspA6 Antibody (OTI1H3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HspA6 Antibody (OTI1H3) - Azide and BSA Free (NBP2-03076) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HSPA6 (NP_002146) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 70.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HspA6 Antibody (OTI1H3) - Azide and BSA Free

• heat shock 70 kDa protein 6
• Heat shock 70 kDa protein B'
• heat shock 70kD protein 6 (HSP70B')
• heat shock 70kDa protein 6 (HSP70B')
• HSP70B'

Background

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA6