HspA4 Antibody (5A6)

Western Blot: HspA4 Antibody (5A6) [NBP1-51592] - Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.

Immunocytochemistry/Immunofluorescence: HspA4 Antibody (5A6) [NBP1-51592] - Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: HspA4 Antibody (5A6) [NBP1-51592] - Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mouse mAb with DAB staining.

Product Discontinued

HspA4 Antibody (5A6) Summary

• Immunogen: Purified recombinant fragment of human HSP70 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:200-1:1000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for HspA4 Antibody (5A6)

• APG2
• APG-2
• heat shock 70 kDa protein 4
• heat shock 70kD protein 4
• heat shock 70kDa protein 4
• Heat shock 70-related protein APG-2
• heat shock protein, 110 kDa
• HS24/P52
• hsp70
• hsp70RY
• MGC131852
• RY

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA4