HSP90 beta Antibody (H9010)

Images

 
Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary ...read more
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal ...read more
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: ...read more
Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. ...read more
Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 ...read more
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: ...read more
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: ...read more
Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Fi, Ha, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, MiAr
Clone
H9010
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSP90 beta Antibody (H9010) Summary

Immunogen
Recombinant human HSP90beta
Localization
Cytoplasm, Melanosome
Marker
ER Stress Marker
Specificity
Detects 90 kDa protein. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:2500
Application Notes
1 ug/ml of HSP90 antibody was sufficient for detection of HSP90beta in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB110-61640 in the following applications:

Publications
Read Publications using
NB110-61640 in the following applications:

  • KD
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP90 beta Antibody (H9010)

  • D6S182
  • Heat shock 84 kDa
  • heat shock 90kD protein 1, beta
  • heat shock 90kDa protein 1, beta
  • heat shock protein 90kDa alpha (cytosolic), class B member 1
  • heat shock protein beta
  • heat shock protein HSP 90-beta
  • HSP 84
  • HSP84
  • HSP90 beta
  • HSP90AB1
  • HSP90B
  • HSP90-BETA
  • HSP90BHSP 90
  • HSPC2
  • HSPC2D6S182
  • HSPCB
  • HSPCBFLJ26984

Background

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta; exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1'2% ofcytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
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Species: Hu, Mu, Rt
Applications: ELISA
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Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
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Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
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Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
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Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
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D6050
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Applications: ELISA
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB

Publications for HSP90 beta Antibody (NB110-61640)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: KD, WB.


Filter By Application
KD
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB110-61640 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018
Yu D, Yao S, Xi W et al. HSC70, HSPA1A, and HSP90AB1 Facilitate Ebola Virus trVLPs to Induce Autophagy bioRxiv Jun 22 2020 (WB, KD, Human) WB, KD Human
Guo B, Xu P, Chai D et al. gB co-immunization with GP96 enhances pulmonary-resident CD8 T cells and exerts a long-term defence against MCMV pneumonitis J Cell Mol Med Nov 6 2020 [PMID: 33155438] (WB, Mouse)

Details:
Western blot analysis of 3T3 cells transfected with pGP96-NT plasmids.
WB Mouse

Reviews for HSP90 beta Antibody (NB110-61640) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using NB110-61640:
Filter by Applications
Western Blot
(3)
Immunohistochemistry-Paraffin
(2)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Western Blot HSP90 beta NB110-61640
Enlarge
5
reviewed by:
Lei Nie
Western Blot Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedHepG2,Human liver cancer cell line (HuH7)
SpeciesHuman
Lot0811
Immunohistochemistry-Paraffin HSP90 beta NB110-61640
Enlarge
5
reviewed by:
Lei Nie
Immunohistochemistry-Paraffin Human 04/04/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
Lot0811
Immunohistochemistry-Paraffin HSP90 beta NB110-61640
Enlarge
5
reviewed by:
Junwei HOU
Immunohistochemistry-Paraffin Human 02/19/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
Lot0811
Western Blot HSP90 beta NB110-61640
Enlarge
4
reviewed by:
Xianghou Xia
Western Blot Human 12/19/2018
View

Summary

ApplicationWestern Blot
Sample Tested293T,hela cell
SpeciesHuman
Lot0811
Western Blot HSP90 beta NB110-61640
Enlarge
5
reviewed by:
Junwei HOU
Western Blot Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedA549 cells,HCC827 human non-small cell lung cancer cell line
SpeciesHuman
Lot0811

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP90 beta Antibody (NB110-61640) (0)

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Lei Nie
04/04/2020
Application: Western Blot
Species: Human

Lei Nie
04/04/2020
Application: Immunohistochemistry-Paraffin
Species: Human

Junwei HOU
02/19/2019
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol HSP90AB1