HSP90 beta Antibody (5G4)

Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of paraffin-embedded kidney cancer tissues (left) and brain tissues (right) using HSP90AB1 mouse mAb with DAB staining.

Flow Cytometry: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green) and negative control (purple).

ELISA: HSP90 beta Antibody (5G4) [NBP2-37590] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 5G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HSP90 beta Antibody (5G4) Summary

• Immunogen: Purified recombinant fragment of human HSP90 beta expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90AB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for HSP90 beta Antibody (5G4)

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP90 beta Antibody (NBP2-37590)(1)

Publication using NBP2-37590

• Marwarha G, Slagsvold KH, Høydal MA NF-?B Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression International journal of molecular sciences 2023-04-01 [PMID: 37047592] (ELISA)

Bioinformatics

• Gene Symbol: HSP90AB1