Human HSP70/HSPA1A ELISA Kit (Colorimetric)

ELISA: Human HSP70/HSPA1A ELISA Kit (Colorimetric) [NBP2-59707] - Typical Standard Curve for the HSP70/HSPA1A ELISA kit (Enzyme-Linked Immunosorbent Assay) - NBP2-59707. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 2.34 - 150 ng/mL.

Product Discontinued

Human HSP70/HSPA1A ELISA Kit (Colorimetric) Summary

• Specificity: This assay is specific for Hsc70 and will not react with Hsp70. The assay does not cross react with 100 ng/mL of Grp94, Hsp60, or Hsp70.
• Standard Curve Range: 2.34 - 150 ng/ml
• Sensitivity: 1.54 ng/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HSPA1A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: ELISA kit used to quantitate HSC70 concentration in samples.
  No of Samples: 40 samples in duplicate
  Incubation Time: 30 minutes

Packaging, Storage & Formulations

• Storage: Store at 4C.

Alternate Names for Human HSP70/HSPA1A ELISA Kit (Colorimetric)

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for **** Hsp70 / Hsc70 ELISA Kit (NBP2-59707). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HSPA1A