HSP70/HSPA1A Antibody (N27F3-4) [Allophycocyanin]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Rb, Sh, Xp
• Applications: WB, Flow, ICC/IF, IHC, IP, MS
• Clone: N27F3-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

HSP70/HSPA1A Antibody (N27F3-4) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant HSP70/HSC70
• Localization: Cytoplasm
• Specificity: Detects approx 72 (HSP) and approx 73kDa (HSC).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-96425. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HSP70/HSPA1A Antibody (N27F3-4) [Allophycocyanin]

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NB110-96425APC)(1)

Publication using NB110-96425APC

• Berner J, Miebach L, Kordt M et al. Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo British journal of cancer 2023-07-17 [PMID: 37460712]

Bioinformatics

• Gene Symbol: HSPA1A
• Entrez:
  • 24472(Rat)