HSP70/HSPA1A Antibody (BRM-22)

Western Blot: HSP70/HSPA1A Antibody (BRM-22) [NB600-1165] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Monoclonal Anti-HSP70 / HSPA1A , Clone: BRM-22 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. A431; 2. A549; 3. NIH-3T3; 4. Hepa 1-6; 5. Mouse brain; 6. Rat brain; 7. MDBK; 8. MDCK; 9. COS-7

Immunohistochemistry: HSP70/HSPA1A Antibody (BRM-22) [NB600-1165] - Staining of formalin-fixed, paraffin-embedded human prostate sections with 1:100 Monoclonal Anti-HSP70 / HSPA1A, Clone: BRM-22 using biotin/ExtrAvidin-Peroxidase.

Flow Cytometry: HSP70/HSPA1A Antibody (BRM-22) [NB600-1165] - A549 cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 1:100 Monoclonal Anti-HSP70 / HSPA1A , Clone: BRM-22 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.

Product Discontinued

HSP70/HSPA1A Antibody (BRM-22) Summary

• Immunogen: Full length native protein (purified) (Bovine brain).
• Localization: Cytoplasmic and Nuclear
• Specificity: By immunoblotting, the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of Hsp70 (BRM-22). The antibody recognizes Hsp70 (BRM-22) from brain and other tissue. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the Hsp70 (BRM-22) protein within the nucleus of heat-shocked (42 degrees C, 1 hour) human fibroblasts.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • ELISA Indirect
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:5000 - 1:10000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human, Rat, Chicken, Cow, Drosophila melanogaster, Guinea Pig, Plants, Rabbit and Nematode. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for HSP70/HSPA1A Antibody (BRM-22)

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an Hsp70 (BRM-22) multigene that differ in their intracellular location and regulation. Members of the Hsp70 (BRM-22) family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. Hsp70 (BRM-22) was found to increase dramatically in the brains of Alzheimer's disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. Hsp70 (BRM-22) concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A
• Entrez:
  • 24472(Rat)