HSP70/HSPA1A Antibody (BRM-22)

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Western Blot: Hsp70 Antibody (BRM-22) [NB600-1165] - Heat Shock Protein 70 antibody in bovine brain lysate [NB600-1165]

Product Details

Summary
Reactivity Hu, Rt, Bv, Ce, Ch, Dr, GP, Pl, RbSpecies Glossary
Applications WB, ELISA, EM, IHC, IHC-Fr
Clone
BRM-22
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP70/HSPA1A Antibody (BRM-22) Summary

Immunogen
Full length native protein (purified) (Bovine brain).
Localization
Cytoplasmic and Nuclear
Specificity
By immunoblotting, the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of Hsp70 (BRM-22). The antibody recognizes Hsp70 (BRM-22) from brain and other tissue. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the Hsp70 (BRM-22) protein within the nucleus of heat-shocked (42 degrees C, 1 hour) human fibroblasts.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Electron Microscopy 1:10-1:500
  • ELISA Indirect
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human, Rat, Chicken, Cow, Drosophila melanogaster, Guinea Pig, Plants, Rabbit and Nematode. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for HSP70/HSPA1A Antibody (BRM-22)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an Hsp70 (BRM-22) multigene that differ in their intracellular location and regulation. Members of the Hsp70 (BRM-22) family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. Hsp70 (BRM-22) was found to increase dramatically in the brains of Alzheimer's disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. Hsp70 (BRM-22) concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSPA1A
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