HSP70/HSPA1A Antibody (3A3)

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Western Blot: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa, isolated from various coves in Rhode Island (indicated above the lanes).
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of Heat Shock Protein 70 (Hsp70) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.
Western Blot: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) shows staining in NIH-3T3 cells. Heat Shock ...read more
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) shows staining in MCF-7 cells. Heat Shock ...read more
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) shows staining in HeLa cells. Heat Shock ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Testis tissue.
Immunoprecipitation: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Immunoprecipitation of Heat Shock Protein 70 (Hsp70) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Av, Dr, Fi, I, Pl, Pm, YeSpecies Glossary
Applications WB, GS, ICC/IF, IHC, IHC-P, IP, ICC, IF
Clone
3A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP70/HSPA1A Antibody (3A3) Summary

Immunogen
Human recombinant HSP70 overexpressed in E. coli.
Localization
Cytoplasmic
Specificity
Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 2 ul
  • Immunocytochemistry 1:100
  • Immunofluorescence 1:50 - 1:200
Application Notes
WB: Detects an approx. proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family.
Publications
Read Publication using
NB600-1469 in the following applications:

  • WB
    1 publication

Reactivity Notes

Arthropod and Mollusca. Insect reactivity reported in scientific literature (PMID: 20798487). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HSP70/HSPA1A Antibody (3A3)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1469
Species: Hu, Mu, Rt, Po, Am, Av, Dr, Fi, I, Pl, Pm, Ye
Applications: WB, GS, ICC/IF, IHC, IHC-P, IP, ICC, IF

Publications for HSP70/HSPA1A Antibody (NB600-1469)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot