HSP27 Antibody

Images

 
Genetic Strategies: Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: Hsp27 Antibody [NBP1-75477] - analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.
Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

HSP27 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot P04792]
Localization
Cytoplasm and nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Predicted Species
Hamster (94%), Canine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockout Validated 2 ug/ml
Application Notes
This HSP27 antibody is useful for ICC/IF, IHC-P, and Western blot where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
Skeletal Muscle Lysate (NB820-59253)
HSP27 Lysate (NBP2-64653)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-75477 in the following applications:

Publications
Read Publications using
NBP1-75477 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human, mouse and rat. Immunogen has 94% identity to canine and hamster.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP27 Antibody

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Heat shock protein 27 is a molecular chaperone involved in protection of cells during cellular stress events. The activity of HSP27 protects from apoptotic events caused by heat shock and other distress. Immunohistochemical detection of HSP27 has been suggested as a qualitative biomarker for colorectal cancer. It is also highly expressed in lung cancer, cerebral inflammation, ischemia-reperfusion injury, and glaucoma. Interestingly, HSP27 overexpression can counteract the activity of multi-drug resistance proteins in breast cancer. Polyphenols downregulate the expression of HSP27, as well as many antioxidant enzymes, resulting in susceptibility to stress and damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP27 Antibody (NBP1-75477)(2)

Review for HSP27 Antibody (NBP1-75477) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-75477:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot HSP27 NBP1-75477
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from Jurkat cells, in lysis buffer
SpeciesHuman
CommentsThe antibody gives a faint band after a very long exposure, which is the case with most other HSP27 antibodies, because of the low levels of HSPs in cells

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:10000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 2 min

Comments

CommentsThe antibody gives a faint band after a very long exposure, which is the case with most other HSP27 antibodies, because of the low levels of HSPs in cells

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP1-75477) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-75477AF405
Alexa Fluor 488 Labeled NBP1-75477AF488
Alexa Fluor 647 Labeled NBP1-75477AF647
Alexa Fluor 700 Labeled NBP1-75477AF700
Biotin Labeled NBP1-75477B
DyLight 405 Labeled NBP1-75477V
DyLight 488 Labeled NBP1-75477G
DyLight 550 Labeled NBP1-75477R
DyLight 650 Labeled NBP1-75477C
FITC Labeled NBP1-75477F
HRP Labeled NBP1-75477H

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Bioinformatics Tool for HSP27 Antibody (NBP1-75477)

Discover related pathways, diseases and genes to HSP27 Antibody (NBP1-75477). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for HSP27 Antibody (NBP1-75477)

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Blogs on HSP27.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

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Recent Reviews

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Anonymous
10/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPB1
Entrez
Uniprot