Home » HSP27 » HSP27 Antibodies » HSP27 Antibody - BSA Free

HSP27 Antibody - BSA Free

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Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Genetic Strategies: Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.
Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-75477AF350
Alexa Fluor 405
NBP1-75477AF405
Alexa Fluor 488
NBP1-75477AF488
Alexa Fluor 532
NBP1-75477AF532
Alexa Fluor 594
NBP1-75477AF594
Alexa Fluor 647
NBP1-75477AF647
Alexa Fluor 700
NBP1-75477AF700
Alexa Fluor 750
NBP1-75477AF750
Biotin
NBP1-75477B
DyLight 350
NBP1-75477UV
DyLight 405
NBP1-75477V
DyLight 488
NBP1-75477G
DyLight 550
NBP1-75477R
DyLight 594
NBP1-75477DL594
DyLight 650
NBP1-75477C
DyLight 680
NBP1-75477FR
DyLight 755
NBP1-75477IR
FITC
NBP1-75477F
HRP
NBP1-75477H
Janelia Fluor 549
NBP1-75477JF549
Janelia Fluor 646
NBP1-75477JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-75477SS
100% Guarantee on every product

HSP27 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot# P04792]
Localization
Cytoplasm and nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Predicted Species
Porcine (94%), Bovine (95%), Hamster (95%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockout Validated
  • Western Blot 2 ug/ml
Application Notes
This HSP27 antibody is useful for ICC/IF, IHC-P, and Western blot where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-75477 in the following applications:
Publications
Read Publications using
NBP1-75477 in the following applications:

  • IHC-P
    1 publication
  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP27 Antibody - BSA Free

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Heat shock protein 27 is a molecular chaperone involved in protection of cells during cellular stress events. The activity of HSP27 protects from apoptotic events caused by heat shock and other distress. Immunohistochemical detection of HSP27 has been suggested as a qualitative biomarker for colorectal cancer. It is also highly expressed in lung cancer, cerebral inflammation, ischemia-reperfusion injury, and glaucoma. Interestingly, HSP27 overexpression can counteract the activity of multi-drug resistance proteins in breast cancer. Polyphenols downregulate the expression of HSP27, as well as many antioxidant enzymes, resulting in susceptibility to stress and damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of Hela cells with HSPA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
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BSA Free
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Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Simple Western: HSP60 Antibody [NBP1-77397] - Lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(4 Reviews)
(3 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
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Simple Western: HSP90 alpha Antibody [NBP1-77685] - Image shows a specific band for Hsp90A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77685] - HSP901a antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: HSP90 alpha Antibody [NBP1-77685] - Staining of human kidney carcinoma in HSP901A using DAB with hematoxylin counterstain.
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
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Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
(1 Review)
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Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38 alpha knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p38 alpha at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Orexin B, p38 alpha , and Integrin  beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin  beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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(5 Publications)
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HSP70/HSPA1A
[Biotin]
DYC1663-2
Lysates prepared from heat-shocked, human Jurkat cells were incubated in wells coated with HSP-70 Duoset IC Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured proteins were electrophoresed, transferred to Immubilon membranes and immunoblotted with HSP-70 Duoset IC Detection Antibody. Only a single band corresponding to HSP-70 was detected.
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Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
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Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in MCF-7 cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 C, washed with PBS and incubated with a DyLight-488 conjugated.
(2 Reviews)
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(31A1067) - (Pro and ...
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Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
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Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
POLDIP2 Antibody
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Western Blot: POLDIP2 Antibody [NBP1-68874] - Staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
Crk Antibody
(3G11E8)
NBP2-37568
ELISA: Crk Antibody (3G11E8) [NBP2-37568] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)
Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - Analysis of Jurkat cells, using anti-GRAP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRAP2 antibody, and then analyzed by flow cytometry.
Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot: AHSA1 Antibody (1A2-A8) [H00010598-M01] - AHSA1 monoclonal antibody (M01), clone 1A2-A8 Analysis of AHSA1 expression in HepG2.
Sandwich ELISA: AHSA1 Antibody (1A2-A8) [H00010598-M01] - Detection limit for recombinant GST tagged AHSA1 is 3 ng/ml as a capture antibody.
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAPKAPK2 Antibody
(2B3)
H00009261-M01
Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of protein-protein interactions between AKT1 and MAPKAPK2. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MAPKAPK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
(1 Review)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, Simple Western, WB

Publications for HSP27 Antibody (NBP1-75477)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
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(1)
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Review for HSP27 Antibody (NBP1-75477) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-75477:
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(1)
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Images Ratings Applications Species Date Details
Western Blot HSP27 NBP1-75477
Enlarge 		3
reviewed by:
Verified Customer
 WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from Jurkat cells, in lysis buffer
SpeciesHuman
CommentsThe antibody gives a faint band after a very long exposure, which is the case with most other HSP27 antibodies, because of the low levels of HSPs in cells

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:10000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 2 min

Comments

CommentsThe antibody gives a faint band after a very long exposure, which is the case with most other HSP27 antibodies, because of the low levels of HSPs in cells

Product General Protocols

View specific protocols for HSP27 Antibody (NBP1-75477): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP1-75477) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

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Western Blot HSP27 NBP1-75477
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 Verified Customer
10/07/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol HSPB1
Entrez
Uniprot