AHSA1 Antibody - BSA Free

Western Blot: AHSA1 Antibody [NBP2-15311] - Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N2C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: AHSA1 Antibody [NBP2-15311] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE AHA-1 antibody, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: AHSA1 Antibody [NBP2-15311] - AHSA1 Antibody detects AHSA1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AHSA1 stained by AHSA1 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: AHSA1 Antibody [NBP2-15311] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Immunocytochemistry/Immunofluorescence: AHSA1 Antibody [NBP2-15311] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AHSA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit AHSA1 Antibody - BSA Free (NBP2-15311) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AHSA1. The exact sequence is proprietary.
• Localization: Cytoplasm; cytosol; Endoplasmic reticulum
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AHSA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:30000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AHSA1 Antibody - BSA Free

• activator of 90 kDa heat shock protein ATPase homolog 1
• AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• C14orf3
• chromosome 14 open reading frame 3
• p38AHA1

Background

Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AHSA1
• Uniprot:
  • O95433(Human)