HSD3B1 Antibody (3C11-D4) - Azide and BSA Free

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - Analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification.Antibody concentration 3 ug/ml. ( a ) Stomach ( b ) Esophagus ( c ) Endometrium ( d ) Uterine cervix ( e ) Placenta ( f ) Ovary, clear cell carcinoma ( g ) Hepatocellular carcinoma ( h ) Breast cancer ( i ) Colon adenocarcinoma ( j and k ) Cervical carcinoma ( l, m, and n ) Choriocarcinoma ( o ) Epithelioid trophoblastic tumor.

Western Blot: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Analysis of HSD3B1 expression in human placenta.

Immunohistochemistry-Paraffin: HSD3B1 Antibody (3C11-D4) [H00003283-M01] - Analysis of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3C11-D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSD3B1 Antibody (3C11-D4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ
• Marker: Trophoblast-associated Marker
• Specificity: HSD3B1 - hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD3B1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSD3B1 Antibody (3C11-D4) - Azide and BSA Free

• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
• 3-beta-HSD I
• 3BETAHSD
• 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
• 3BH
• delta-5-3-ketosteroid isomerase
• EC 1.1.1
• HSD3B
• HSDB3
• HSDB3A
• hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
• I
• progesterone reductase
• SDR11E1
• short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
• steroid Delta-isomerase
• Trophoblast antigen FDO161G

Background

3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase (HSD3B1) is a bifunctional enzyme involved in the oxidative conversion of steroids and plays an important role in the synthesis of all steroid hormones. There are two HSD3B1 proteins--designated type I and type II--that are expressed by different genes and function in different areas of the body. HSD3B1 has also been shown to be a highly specific and sensitive trophoblast-associated marker.

HSD3B1 is required for the production of progesterone by the placenta, so it is predicted that mutations in this gene may cause infertility in women. HSD3B1 antibodies are useful tools for researchers studying hormone regulation and reproductive biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSD3B1 Antibody (H00003283-M01)(8)

Publications using H00003283-M01

• Xing, D;Zheng, G;Pallavajjala, A;Schoolmeester, JK;Liu, Y;Haley, LM;Hu, Y;Liu, L;Logan, LS;Lin, Y;Pearce, KE;Sattler, CA;Tsai, YC;Vang, R;Hung, CF;Wu, TC;Ronnett, BM; Lineage-specific alterations in gynecologic neoplasms with choriocarcinomatous differentiation: implications for origin and therapeutics Clin. Cancer Res. 2019-04-22 [PMID: 31010836] (IHC-P, Human)
• Ito A, Yamazaki Y, Sasano H et al. A case of primary aldosteronism caused by unilateral multiple adrenocortical micronodules presenting as muscle cramps at rest: The importance of functional histopathology for identifying a culprit lesion. Pathol Int 2017-03-05 [PMID: 28261922]
• Kubota F, Nakamura Y, Konosu-Fukaya S et al. Expression of steroidogenic enzymes and their transcription factors in cortisol-producing adrenocortical adenomas: immunohistochemical analysis and quantitative RT-PCR studies. Hum Pathol 2016-04-13 [PMID: 27085553]
• Azmahani A, Nakamura Y, Felizola SJ et al. Steroidogenic enzymes, their related transcription factors and nuclear receptors in human sebaceous glands under normal and pathological conditions. J Steroid Biochem Mol Biol. 2014-07-29 [PMID: 25090634]
• Mao TL, Shih IM. Advances in the diagnosis of gestational trophoblastic tumors and tumor-like lesions. Expert Opin. Med. Diagn. 2009-01-01 [PMID: 23485206]
• Ariel I, Boldes R, Weintraub A et al. Chorangiocarcinoma: A Case Report and Review of the Literature. Int J Gynecol Pathol. 2009-05-01 [PMID: 19620945]
• Mao TL, Kurman RJ, Jeng YM et al. HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors tumorlike lesions. Am J Surg Pathol;32(2):236-42. 2008-02-01 [PMID: 18223326]
• Shih IeM. Trophogram, anIHC-based algorithmic approach, in the differential diagnosis of trophoblastic tumors tumorlike lesions. Ann Diagn Pathol;11(3):228-34. 2007-06-01 [PMID: 17498600]

Bioinformatics

• Gene Symbol: HSD3B1
• Entrez:
  • 3283(Human)
• OMIM:
  • 109715(Human)
• Uniprot:
  • AAH31999(Human)