HSD17B2 Antibody (OTI4F10)

Western Blot: HSD17B2 Antibody (4F10) [NBP2-01952] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B2.

Flow Cytometry: HSD17B2 Antibody (4F10) [NBP2-01952] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSD17B2 antibody, and then analyzed by flow cytometry.

Flow Cytometry: HSD17B2 Antibody (4F10) [NBP2-01952] - Analysis of Jurkat cells, using anti-HSD17B2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Simple Western, Flow
• Clone: OTI4F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.45 mg/ml

HSD17B2 Antibody (OTI4F10) Summary

• Description: Novus Biologicals Mouse HSD17B2 Antibody (OTI4F10) (NBP2-01952) is a monoclonal antibody validated for use in WB, Flow and Simple Western. Anti-HSD17B2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HSD17B2(NP_002144) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD17B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Simple Western
  • Western Blot 1:2000
• Application Notes: See Simple Western Antibody Database for Simple Western validation
• Theoretical MW: 42.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.45 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSD17B2 Antibody (OTI4F10)

• 17-beta-hydroxysteroid dehydrogenase type 2
• 20 alpha-hydroxysteroid dehydrogenase
• E2DH
• EC 1.1.1.62,17-beta-HSD 2
• EC 1.1.1.63
• EDH17B220-alpha-HSD
• estradiol 17-beta-dehydrogenase 2
• HSD17
• hydroxysteroid (17-beta) dehydrogenase 2
• Microsomal 17-beta-hydroxysteroid dehydrogenase
• SDR9C2
• short chain dehydrogenase/reductase family 9C, member 2
• Testosterone 17-beta-dehydrogenase

Background

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol andestrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSD17B2 Antibody (NBP2-01952)(1)

Publication using NBP2-01952

• Hill JL, McIver KB, Katzer K, Foster MT Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue Methods and protocols 2022-04-15 [PMID: 35448699] (WB, Simple Western, Human)

Bioinformatics

• Gene Symbol: HSD17B2
• Entrez:
  • 3294(Human)