HO-1/HMOX1/HSP32 Antibody [Unconjugated]

Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HO-1/HMOX1/HSP32 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat HO-1/HMOX1
  Met1-Met289
  Accession # P06762
• Specificity: Detects mouse and rat HO-1/HMOX1/HSP32 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMOX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HO-1/HMOX1/HSP32 Antibody [Unconjugated]

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Rat HO-1 shares 93% amino acid sequence identity with mouse HO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-1/HMOX1/HSP32 Antibody (AF3169)(4)

Publications using AF3169

• MS Abdelfatta, MIY Elmallah, HY Ebrahim, RS Almeer, RMA Eltanany, AE Abdel Mone Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms PLoS ONE, 2019-06-13;14(6):e0216737. 2019-06-13 [PMID: 31194753] (Western Blot, Rat)
• RS Almeer, GI AlBasher, S Alarifi, S Alkahtani, D Ali, AE Abdel Mone Royal jelly attenuates cadmium-induced nephrotoxicity in male mice Sci Rep, 2019-04-09;9(1):5825. 2019-04-09 [PMID: 30967588] (Western Blot, Mouse)
• Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling International Journal of Molecular Medicine 12/12/2018 [PMID: 30569096]
• Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke Evid Based Complement Alternat Med, 2016-11-28;2016(0):1698379. 2016-11-28 [PMID: 28003846] (Western Blot, Rat)

Review for HO-1/HMOX1/HSP32 Antibody (AF3169) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21546574
• Species: Rat

Bioinformatics

• Gene Symbol: HMOX1
• Uniprot:
  • P06762()