Western Blot: hnRNP U Antibody [NB100-2134] - Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody used for WB ...read more
Immunohistochemistry-Paraffin: hnRNP U Antibody [NB100-2134] - Human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-U used at a dilution of 1:200 (1 ...read more
Western Blot: hnRNP U Antibody [NB100-2134] - Detection of human hnRNP-U by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified ...read more
Simple Western: hnRNP U Antibody [NB100-2134] - Electropherogram from Simple Western (WES) of HNRNPU (NB100-2134). Antibody at 1:100 on protein sample from Neural Epithelial Stem Cells. Simple Western image submitted by ...read more
Immunoprecipitation: Not Recommended, use rabbit anti-hnRNP-U antibody NB100-2135. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. This hnRNP U antibody is validated for Simple Western from a verified customer review.
heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
heterogeneous nuclear ribonucleoprotein U
p120 nuclear protein
Scaffold attachment factor A
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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