hnRNP U Antibody

Images

 
Western Blot: hnRNP U Antibody [NB100-2134] - Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody used for WB ...read more
Immunohistochemistry-Paraffin: hnRNP U Antibody [NB100-2134] - Human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-U used at a dilution of 1:200 (1 ...read more
Western Blot: hnRNP U Antibody [NB100-2134] - Detection of human hnRNP-U by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified ...read more
Simple Western: hnRNP U Antibody [NB100-2134] - Electropherogram from Simple Western (WES) of HNRNPU (NB100-2134). Antibody at 1:100 on protein sample from Neural Epithelial Stem Cells. Simple Western image submitted by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P, IP, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

hnRNP U Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human Heterogeneous Nuclear Ribonucleoprotein U using the numbering given in entry NP_114032.1 (GeneID 3192).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:2000 - 1:10000
Application Notes
Immunoprecipitation: Not Recommended, use rabbit anti-hnRNP-U antibody NB100-2135. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. This hnRNP U antibody is validated for Simple Western from a verified customer review.
Control
hnRNP U Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-2134 in the following applications:

Publications
Read Publication using NB100-2134.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP U Antibody

  • heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
  • heterogeneous nuclear ribonucleoprotein U
  • hnRNP U
  • HNRPU
  • p120 nuclear protein
  • p120
  • pp120
  • SAFA
  • SAF-AhnRNPU
  • Scaffold attachment factor A
  • U21.1

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67327
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-12793
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP2-67330
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
PP-H4417-00
Species: Hu
Applications: DirELISA, IP, WB
NBP1-76869
Species: Hu
Applications: ELISA, ICC/IF, WB
AF2558
Species: Hu, Mu
Applications: Simple Western, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
H00004068-M01
Species: Hu, Mu
Applications: ELISA, Flow, Func, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB600-241
Species: Hu, Mu, Pl, Rt
Applications: ChIP, EM, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IM, KD, Simple Western, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-38806
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-67427
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
236-EG
Species: Hu
Applications: BA

Publications for hnRNP U Antibody (NB100-2134)(1)

Review for hnRNP U Antibody (NB100-2134) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2134:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western hnRNP U NB100-2134
Enlarge
4
reviewed by:
Francesca Mastropasqua
Simple Western Human 11/28/2019
View

Summary

ApplicationSimple Western
Sample TestedHuman fibroblast and neural stem cells
SpeciesHuman
LotA1

Comments

CommentsDilution 1:100

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for hnRNP U Antibody (NB100-2134) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional hnRNP U Products

Bioinformatics Tool for hnRNP U Antibody (NB100-2134)

Discover related pathways, diseases and genes to hnRNP U Antibody (NB100-2134). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for hnRNP U Antibody (NB100-2134)

Discover more about diseases related to hnRNP U Antibody (NB100-2134).
 

Pathways for hnRNP U Antibody (NB100-2134)

View related products by pathway.

Research Areas for hnRNP U Antibody (NB100-2134)

Find related products by research area.

Blogs on hnRNP U

There are no specific blogs for hnRNP U, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Francesca Mastropasqua
11/28/2019
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol HNRNPU
Entrez
Uniprot