hnRNP H Antibody

Images

 
Western Blot: hnRNP H Antibody [NBP1-31648] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP1-31648] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NBP1-31648] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:500.Red: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

hnRNP H Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human hnRNP H. The exact sequence is proprietary.
Localization
Nucleus, nucleoplasm
Predicted Species
Rat (99%), Xenopus (91%), Bovine (100%), Chicken (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP H Antibody

  • DKFZp686A15170
  • heterogeneous nuclear ribonucleoprotein H
  • heterogeneous nuclear ribonucleoprotein H1 (H)
  • hnRNP H
  • HNRPH
  • HNRPH1hnRNPH

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HNRNPH1