HNF-3 alpha/FoxA1 Antibody

Images

 
Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Paraffin-embedded human cervical carcinoma. FOXA1 antibody dilution: 1:500.
Chromatin Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Paraffin-embedded human breast carcinoma. FOXA1 antibody dilution: 1:500.
Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ug whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HNF-3 alpha/FoxA1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (100%), Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Chromatin Immunoprecipitation (ChIP)
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HNF-3 alpha/FoxA1 Lysate (NBP2-66232)

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HNF-3 alpha/FoxA1 Antibody (NBP1-30877) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

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Bioinformatics

Gene Symbol FOXA1