HNF-3 alpha/FoxA1 Antibody (S04-5A7)

Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Western blot detection of HNF-3 alpha/FoxA1 in C6 cell lysates using NBP3-19637 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Immunofluorescence of HNF-3 alpha/FoxA1 (green) in MCF-7 using NBP3-19637 at dilution 1/20, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S04-5A7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

HNF-3 alpha/FoxA1 Antibody (S04-5A7) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human HNF-3 alpha/FoxA1 (Uniprot # P55317)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FOXA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20
  • Western Blot 1/1000-1/5000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (S04-5A7)

• forkhead box A1
• Forkhead box protein A1
• FoxA1
• hepatocyte nuclear factor 3, alpha
• hepatocyte nuclear factor 3-alpha
• HNF3 alpha
• HNF-3 alpha
• HNF-3A
• HNF-3-alpha
• HNF3ATCF-3A
• MGC33105
• TCF3A
• Transcription factor 3A

Background

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXA1