HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Immunoprecipitating HNF-3 alpha/FoxA1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26572 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26572 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Positive Western Blot detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate. All lanes: HNF-3 alpha/FoxA1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 46 kDa Observed band size: 50 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 3B2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human HNF-3 alpha/FoxA1 [UniProt P55317]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FOXA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (3B2) - BSA Free

• forkhead box A1
• Forkhead box protein A1
• FoxA1
• hepatocyte nuclear factor 3, alpha
• hepatocyte nuclear factor 3-alpha
• HNF3 alpha
• HNF-3 alpha
• HNF-3A
• HNF-3-alpha
• HNF3ATCF-3A
• MGC33105
• TCF3A
• Transcription factor 3A

Background

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXA1