HMGB1/HMG-1 Antibody - BSA Free

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Mouse Tissues.Loading: 15 ul of lysate Antibodies: HMGB1/HMG-1 , 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Rat Tissues.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in HL60 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Human Stomach Tissue.Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 ul/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt, Po, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HMGB1/HMG-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HMGB1/HMG-1 Antibody - BSA Free (NBP2-41264) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid peptide near the center of human HMGB1. The immunogen is located within amino acids 70 - 120 of HMGB1. Amino Acid Squence: KGETKKKFKDPNAPKRPPS
• Predicted Species: Porcine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGB1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20ug/mL
  • Immunohistochemistry 1-2 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 1 ug/mL
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for HMGB1/HMG-1 Antibody - BSA Free

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB1