HMG-CoA Reductase/HMGCR Antibody

Product Discontinued

HMG-CoA Reductase/HMGCR Antibody Summary

• Immunogen: Peptide with sequence C-ADPSPQNSTADTSK corresponding to internal region according to NP_000850.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMGCR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments in human brain (cerebellum), liver, skeletal muscle and adipose lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HMG-CoA Reductase/HMGCR Antibody

• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• EC 1.1.1
• EC 1.1.1.34
• HMG-CoA Reductase
• HMGCR
• Hydroxymethylglutaryl-CoA Reductase
• LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMG-CoA Reductase/HMGCR Antibody (NBP1-36961)(1)

Publication using NBP1-36961

• Hanai J, Cao P, Tanksale P et al. The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity. J Clin Invest 2007-12-01 [PMID: 17992259]

Bioinformatics

• Gene Symbol: HMGCR
• Entrez:
  • 3156(Human)
• Uniprot:
  • P04035()