HLA DPA Antibody

Western Blot: HLA DPA Antibody [NBP2-16851] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: HLA DPA Antibody [NBP2-16851] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: HLA DPA Antibody [NBP2-16851] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HLA DPA Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HLA-DPA1. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLA-DPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for HLA DPA Antibody

• DP alpha 1 chain
• HLA class II histocompatibility antigen, DP alpha chain
• HLADP
• HLA-SB alpha chain
• major histocompatibility complex, class II, DP alpha 1
• MHC class II antigen
• MHC class II DP3-alpha
• MHC class II DPA1
• MHC class II HLA-DPA1 antigen
• Primed lymphocyte test-1

Background

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HLA DPA Antibody (NBP2-16851)(1)

Publication using NBP2-16851

• Li L, Kim H, Park S et al. Genetic Profiles Associated with Chemoresistance in Patient-Derived Xenograft Models of Ovarian Cancer. Cancer Res Treat. 2018-11-06 [PMID: 30428638] (IF/IHC, Human)

FAQs for HLA DPA Antibody (NBP2-16851). (Showing 1 - 1 of 1 FAQ).

1. We are planning to buy the HLA-DPA1 and HLA-DMB Abs from your company to do the IHC in our Tumor samples. We saw the image of the tissue, and my PI wants to know if you can send us the exact protocol you have used to do the IHC for NBP2-16851 and NBP1-85326?
   • These two antibodies should work with any general IHC-P protocol. Our general IHC-P protocol can be found here.

Bioinformatics

• Gene Symbol: HLA-DPA1
• Uniprot:
  • P20036(Human)