HLA Class I Antibody (MEM-147) - BSA Free

Western Blot: HLA Class I Antibody (MEM-147) [NB500-411] - Human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: MEM-147
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA Class I Antibody (MEM-147) - BSA Free Summary

• Immunogen: PHA-activated peripheral blood lymphocytes
• Specificity: This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-5 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Western Blot: Non-reducing conditions. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HLA Class I Antibody (MEM-147) - BSA Free

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A
• Entrez:
  • 3106(Human)
  • 3107(Human)