HLA Class I Antibody (MEM-147) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
MEM-147
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

HLA Class I Antibody (MEM-147) [DyLight 488] Summary

Immunogen
PHA-activated peripheral blood lymphocytes
Specificity
This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HLA Class I Antibody (MEM-147) [DyLight 488]

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLA Class I
  • HLAA
  • HLA-A
  • HLA-ABC
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB500-411APC
DyLight 350 Labeled NB500-411UV
DyLight 405 Labeled NB500-411V
DyLight 680 Labeled NB500-411FR
DyLight 755 Labeled NB500-411IR
PE Labeled NB500-411PE
PerCP Labeled NB500-411PCP

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Bioinformatics

Gene Symbol HLA-A
Entrez