HLA Class I Antibody (MEM-147) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
MEM-147
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350

Order Details

HLA Class I Antibody (MEM-147) [Alexa Fluor® 350] Summary

Immunogen
PHA-activated peripheral blood lymphocytes
Specificity
This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HLA Class I Antibody (MEM-147) [Alexa Fluor® 350]

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLA Class I
  • HLAA
  • HLA-A
  • HLA-ABC
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-411AF350
Alexa Fluor 405 NB500-411AF405
Alexa Fluor 488 NB500-411AF488
Alexa Fluor 532 NB500-411AF532
Alexa Fluor 594 NB500-411AF594
Alexa Fluor 647 NB500-411AF647
Alexa Fluor 700 NB500-411AF700
Alexa Fluor 750 NB500-411AF750
Allophycocyanin NB500-411APC
Allophycocyanin/Cy7 NB500-411APCCY7
DyLight 350 NB500-411UV
DyLight 405 NB500-411V
DyLight 488 NB500-411G
DyLight 550 NB500-411R
DyLight 594 NB500-411DL594
DyLight 650 NB500-411C
DyLight 680 NB500-411FR
DyLight 755 NB500-411IR
Janelia Fluor 549 NB500-411JF549
Janelia Fluor 646 NB500-411JF646
PE NB500-411PE
PE/Atto594 NB500-411PEATT594
PE/Cy5.5 NB500-411PECY55
PE/Cy7 NB500-411PECY7
PerCP NB500-411PCP

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Array NB500-411AF350

Bioinformatics Tool for HLA Class I Antibody (NB500-411AF350)

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Bioinformatics

Gene Symbol HLA-A