HLA G Antibody (MEM-G/9)

Images

 
Immunocytochemistry/ Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor 488 Fab-fragment.
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and ...read more
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of FITC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human ...read more
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.
Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Detection of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, CyTOF-ready
Clone
MEM-G/9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HLA G Antibody (MEM-G/9) Summary

Immunogen
Recombinant human HLA-G refolded with beta2-microglobulin and peptide (NM_002127.5)
Epitope
aa 16-25 of human p53
Localization
Cell Membrane
Specificity
The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-G
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:2000
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 ug/ml), detection antibody (biotin or peroxidase conjugate, 1 ug/ml). Purity is > 95% (by SDS-PAGE). Use in western blot reported in scientific literature ( PMID 24054889)
Publications
Read Publications using
NB500-314 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HLA G Antibody (MEM-G/9)

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA G
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • HLA-G
  • major histocompatibility complex, class I, G
  • MHC Class I Antigen G
  • MHC-G

Background

HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA G Antibody (NB500-314)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA G Antibody (NB500-314) (0)

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Secondary Antibodies

 

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Blogs on HLA G.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-G
Entrez