Immunocytochemistry/ Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor 488 Fab-fragment.
Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and ...read more
Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Detection of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was ...read more
Recombinant human HLA-G refolded with beta2-microglobulin and peptide (NM_002127.5)
aa 16-25 of human p53
The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Protein A purified
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ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 ug/ml), detection antibody (biotin or peroxidase conjugate, 1 ug/ml). Purity is > 95% (by SDS-PAGE). Use in western blot reported in scientific literature ( PMID 24054889)
HLA class I histocompatibility antigen, alpha chain G
HLA class I molecule
HLA G antigen
HLA-G histocompatibility antigen, class I, G
major histocompatibility complex, class I, G
MHC class I antigen G
HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.