HLA A Antibody

Images

 
Western Blot: HLA A Antibody [NBP2-14982] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit ...read more
Immunohistochemistry-Paraffin: HLA A Antibody [NBP2-14982] - Human tonsil. HLA-A stained by HLA-A antibody [N1C2] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: HLA A Antibody [NBP2-14982] - IP samples: A549 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 ug HLA-A antibody [N1C2] 10 % SDS-PAGE The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HLA A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HLA-A. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLA-A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry -Assay dependent
  • Immunohistochemistry-Paraffin -Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Application Notes
IHC-P-Assay dependent.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HLA A Antibody

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLAA
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-A