Histone H4 [Trimethyl Lys20] Antibody

Images

 
Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.
Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.
Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Simple Western, DB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.58 mg/ml

Order Details

Histone H4 [Trimethyl Lys20] Antibody Summary

Immunogen
Synthetic trimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot# P62805].
Modification
Trimethyl Lys20
Localization
Nucleus
Marker
H4K20me3
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Dot Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 23924899). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23924899).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
Histone H4 Peptide (NB21-2090PEP)
Publications
Read Publication using
NB21-2090 in the following applications:

Reactivity Notes

Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.58 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H4 [Trimethyl Lys20] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K20Me3
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NB21-2090)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC-IF, WB.


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(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2090) (0)

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Secondary Antibodies

 

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot