Histone Deacetylase 4/HDAC4 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Histone Deacetylase 4/HDAC4 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Histone Deacetylase 4/HDAC4
  Met1-Gln68
  Accession # P56524
• Specificity: Detects human, mouse, and rat Histone Deacetylase 4/HDAC4 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone Deacetylase 4/HDAC4 Antibody [Alexa Fluor® 405]

• HA6116
• HD4EC 3.5.1.98
• HDAC4
• HDAC-A
• HDACABDMR
• Histone Deacetylase 4
• histone deacetylase A
• KIAA0288AHO3

Background

Histone Deacetylase 4 (HDAC4; also HD4) is a founding member of the class IIa subfamily, histone deacetylase family of transcriptional regulators. Although its predicted MW is 119 kDa, it runs anomalously at 140‑150 kDa in SDS‑PAGE which may be due to extensive phosphorylation plus SUMOylation. It has an N-terminal region that interacts with transcription factors and corepressors, and a C-terminal domain that shows deacetylase activity, thus repressing gene transcription. HDAC4 is found in osteoblasts, cardiac and skeletal muscle cells, and neurons. Human HDAC4 is 1084 amino acids (aa) in length. It contains one coiled-coil region (aa 67‑177), a SUMOylation site at Lys559, a histone deacetylation domain (aa 665‑993) and one NES (aa 1051‑1084). Caspase cleavage after Asp289 generates bioactive 97 and 34 kDa fragments. There is one potential splice variant that shows an alternative start site at Met118 coupled to a five aa insertion after Thr431. Over aa 1‑68, human HDAC4 shares 96% aa identity with mouse HDAC4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.