HIF Prolyl Hydroxylases Antibody Pack

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.

Immunohistochemistry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Staining of renal tubular epithelium in mouse at 2.5ug/ml. Mouse kidney cortex, 40X.

Flow Cytometry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red).

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 (NB100-139) use at 1:1,000 (A) or 1:5,000 (B). Detection: Chemiluminence.

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD4 Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD3. Samples: HeLa whole cell lysates and MEFs.

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD.

Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.

Immunohistochemistry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Staining of lung, vascular endothelium.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
• Host: Rabbit
• Concentration: Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

HIF Prolyl Hydroxylases Antibody Pack Summary

• Immunogen: The exact amino acid range is proprietary. General Immunogen area:
  NB100-137 (LocusLink - 54583 Region - N-Terminus),
  NB100-138 (LocusLink - 54583 Region - C-Terminus),
  NB100-139 (LocusLink - 112399 Region - Internal),
  NB100-295 (LocusLink - 54681 Region - c-terminus),
  NB100-303 (LocusLink - 112399 Region - c-terminus),
  NB100-2219 (mouse amino acid range 300-400).
• Specificity: See individual datasheets.
• Host: Rabbit
• Gene: EGLN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Application Notes: These antibodies can be used for Western Blot at a dilution range of 1:500~1:1,000. Other applications, such as immunofluorescence, are not confirmed.
• Positive Control:

  EGLN1/PHD2 Lysate (NBL1-10153)

Reactivity Notes

All: Homo sapiens.
NB100-137 does not react with mouse.
NB100-138 does not react with mouse and mixed results in rat.
NB100-139 does not react with mouse but reacts with rat.
NB100-295 does not react with mouse and is unknown in rat.
NB100-303 reacts with mouse.
Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: 0.01% Sodium Azide
• Concentration: Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
• Purity: Immunogen affinity purified

Notes

1 vial each of: NB100-2219SS (0.025ml), NB100-303SS (0.025ml), NB100-139SS (0.025ml), NB100-138SS (0.025ml), NB100-295SS (0.025ml) and NB100-137SS (0.025ml).

Alternate Names for HIF Prolyl Hydroxylases Antibody Pack

• C1orf12HPH2
• EC 1.14.11
• EC 1.14.11.-
• ECYT3
• egl nine homolog 1 (C. elegans)
• egl nine homolog 1
• egl nine-like protein 1
• HIF prolyl hydroxylase 2
• HIF-PH2
• HIFPH2EGL nine (C.elegans) homolog 1
• HIF-prolyl hydroxylase 2
• HPH-2
• Hypoxia-inducible factor prolyl hydroxylase 2
• PHD2SM20
• Prolyl hydroxylase domain-containing protein 2
• SM-20
• zinc finger MYND domain-containing protein 6
• ZMYND6DKFZp761F179

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for HIF Prolyl Hydroxylases Antibody Pack (NB100-903PHD)(15)

Publications using NB100-903PHD

• Siddiq A, Aminova LR, Troy CM et al. Selective Inhibition of Hypoxia-Inducible Factor (HIF) Prolyl-Hydroxylase 1 Mediates Neuroprotection against Normoxic Oxidative Death via HIF- CREB-Independent Pathways. J Neurosci;29(27):8828-8838. 2009 [PMID: 19587290]
• Loinard C, Ginouves A, Vilar J et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation;120(1):50-59. 2009 [PMID: 19546390]
• Schultz K, Murthy V, Tatro JB et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol;296(6):L921-927. 2009 [PMID: 19304911]
• Mikhaylova, O, Ignacak, M et al. The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress. Mol Cell Biol;28(8):2701-17. 2008 Apr. [PMID: 18285459]
• Chang, E et al. Age decreases endothelial progenitor cell recruitment through decreases in hypoxia-inducible factor 1alpha stabilization during ischemia. Circulation;116(24):2818-29. 2007 Dec 11. [PMID: 18040029]
• Bex, C et al. A yeast two-hybrid system reconstituting substrate recognition of the von Hippel-Lindau tumor suppressor protein. Nucleic Acids Res;35(21):e142. 2007 [PMID: 17986458]
• Baek, JH et al. Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha. J Biol Chem;282(46):33358-66. 2007 Nov 16. [PMID: 17875644]
• Colla S, Tagliaferri S, Morandi F et al. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis. Blood;110(13):4464-75. 2007 Dec 15. [PMID: 17848618]
• Baek, JH et al. Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha. J Biol Chem;282(32):23572-80. 2007 Aug 10. [PMID: 17558023]
• Choi, Y et al. AKAP12 regulates human blood-retinal barrier formation by downregulation of hypoxia-inducible factor-1alpha. J Neurosci;27(16):4472-81. 2007 Apr 18. [PMID: 17442832]
• Grover, TR et al. Hypoxia-inducible factors HIF-1alpha and HIF-2alpha are decreased in an experimental model of severe respiratory distress syndrome in preterm lambs. Am J Physiol Lung Cell Mol Physiol;292(6):L1345-51. 2007 Jun. [PMID: 17307811]
• Kondo, S et al. EBV latent membrane protein 1 up-regulates hypoxia-inducible factor 1alpha through Siah1-mediated down-regulation of prolyl hydroxylases 1 and 3 in nasopharyngeal epithelial cells. Cancer Res;66(20):9870-7. 2006 Oct 15. [PMID: 17047048]
• McMahon, S et al. Transforming growth factor beta1 induces hypoxia-inducible factor-1 stabilization through selective inhibition of PHD2 expression. J Biol Chem;281(34):24171-81. 2006 Aug 25. [PMID: 16815840]
• Stiehl, DP et al. Increased prolyl 4-hydroxylase domain proteins compensate for decreased oxygen levels. Evidence for an autoregulatory oxygen-sensing system. J Biol Chem;281(33):23482-91. 2006 Aug 18. [PMID: 16790428]
• Lu H et al. Reversible inactivation of HIF-1 prolyl hydroxylases allows cell metabolism to control basal HIF-1. J Biol Chem;280(51):41928-39. 2005 Dec 23. [PMID: 16223732]

Bioinformatics

• Gene Symbol: EGLN1