HIF Prolyl Hydroxylases Antibody Pack

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Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.
Immunohistochemistry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Staining of renal tubular epithelium in mouse at 2.5ug/ml. Mouse kidney cortex, 40X.
Flow Cytometry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype ...read more
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 ...read more
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD4 Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD3. Samples: HeLa whole cell lysates and MEFs.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.
Immunohistochemistry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Staining of lung, vascular endothelium.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Host
Rabbit
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Order Details

HIF Prolyl Hydroxylases Antibody Pack Summary

Immunogen
The exact amino acid range is proprietary. General Immunogen area:
NB100-137 (LocusLink - 54583 Region - N-Terminus),
NB100-138 (LocusLink - 54583 Region - C-Terminus),
NB100-139 (LocusLink - 112399 Region - Internal),
NB100-295 (LocusLink - 54681 Region - c-terminus),
NB100-303 (LocusLink - 112399 Region - c-terminus),
NB100-2219 (mouse amino acid range 300-400).
Specificity
See individual datasheets.
Host
Rabbit
Gene
EGLN1
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
These antibodies can be used for Western Blot at a dilution range of 1:500~1:1,000. Other applications, such as immunofluorescence, are not confirmed.
Positive Control
EGLN1/PHD2 Lysate (NBL1-10153)
Publications
Read Publications using NB100-903PHD.

Reactivity Notes

All: Homo sapiens.
NB100-137 does not react with mouse.
NB100-138 does not react with mouse and mixed results in rat.
NB100-139 does not react with mouse but reacts with rat.
NB100-295 does not react with mouse and is unknown in rat.
NB100-303 reacts with mouse.
Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Preservative
0.01% Sodium Azide
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Purity
Immunogen affinity purified

Kit Components

Notes

1 vial each of: NB100-2219SS (0.025ml), NB100-303SS (0.025ml), NB100-139SS (0.025ml), NB100-138SS (0.025ml), NB100-295SS (0.025ml) and NB100-137SS (0.025ml).

Alternate Names for HIF Prolyl Hydroxylases Antibody Pack

  • C1orf12HPH2
  • EC 1.14.11
  • EC 1.14.11.-
  • ECYT3
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • egl nine-like protein 1
  • HIF prolyl hydroxylase 2
  • HIF-PH2
  • HIFPH2EGL nine (C.elegans) homolog 1
  • HIF-prolyl hydroxylase 2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2SM20
  • Prolyl hydroxylase domain-containing protein 2
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6DKFZp761F179

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NB100-903PHD
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for HIF Prolyl Hydroxylases Antibody Pack (NB100-903PHD)(15)


Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-903PHD Applications Species
Siddiq A, Aminova LR, Troy CM et al. Selective Inhibition of Hypoxia-Inducible Factor (HIF) Prolyl-Hydroxylase 1 Mediates Neuroprotection against Normoxic Oxidative Death via HIF- CREB-Independent Pathways. J Neurosci;29(27):8828-8838. 2009 [PMID: 19587290]
Loinard C, Ginouves A, Vilar J et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation;120(1):50-59. 2009 [PMID: 19546390]
Schultz K, Murthy V, Tatro JB et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol;296(6):L921-927. 2009 [PMID: 19304911]
Mikhaylova, O, Ignacak, M et al. The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress. Mol Cell Biol;28(8):2701-17. 2008 Apr. [PMID: 18285459]
Chang, E et al. Age decreases endothelial progenitor cell recruitment through decreases in hypoxia-inducible factor 1alpha stabilization during ischemia. Circulation;116(24):2818-29. 2007 Dec 11. [PMID: 18040029]
Bex, C et al. A yeast two-hybrid system reconstituting substrate recognition of the von Hippel-Lindau tumor suppressor protein. Nucleic Acids Res;35(21):e142. 2007 [PMID: 17986458]
Baek, JH et al. Spermidine/spermine N(1)-acetyltransferase-1 binds to hypoxia-inducible factor-1alpha (HIF-1alpha) and RACK1 and promotes ubiquitination and degradation of HIF-1alpha. J Biol Chem;282(46):33358-66. 2007 Nov 16. [PMID: 17875644]
Colla S, Tagliaferri S, Morandi F et al. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis. Blood;110(13):4464-75. 2007 Dec 15. [PMID: 17848618]
Baek, JH et al. Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha. J Biol Chem;282(32):23572-80. 2007 Aug 10. [PMID: 17558023]
Choi, Y et al. AKAP12 regulates human blood-retinal barrier formation by downregulation of hypoxia-inducible factor-1alpha. J Neurosci;27(16):4472-81. 2007 Apr 18. [PMID: 17442832]
Show All 15 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HIF Prolyl Hydroxylases Antibody Pack (NB100-903PHD) (0)

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Positive Control Lysate(s)

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HIF Prolyl Hydroxylases NB100-903PHD

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Blogs on HIF Prolyl Hydroxylases.

HIF Prolyl Hydroxylase 1: A Key Regulator of HIF1A Levels
Hypoxia inducible factors (HIFs) are transcription factors that regulate the cellular response to decreases in oxygen levels. Under conditions of hypoxia HIFs activate the transcription of a diverse range of genes, resulting in increased oxygen delive...  Read full blog post.

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Bioinformatics

Gene Symbol EGLN1