HIF-3 alpha Antibody - BSA Free

Immunohistochemistry: HIF-3 alpha Antibody [NBP1-03155] - Expression of hypoxia-inducible alpha subunits in normal and diseased lung tissue. There is no significant difference in HIF-3alpha expression in fibroblasts; however, in the alveolar wall, the latter shows a positive IHC reaction in different cellular elements in controls (i) and hypersensitivity pneumonitis (h), while no expression in idiopathic pulmonary fibrosis (g). Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-019-1100-4), licensed under a CC-BY licence.

Western Blot: HIF-3 alpha Antibody [NBP1-03155] - (lane 1) 3T3 cell lysate and (lane 2) control 3T3 cell lysate.

Western Blot: HIF-3 alpha Antibody [NBP1-03155] - IPF derived fibroblasts have low expression of HIF-3alpha by hypermethylation. Western blot of HIF3alpha in IPF derived fibroblasts after treatment with 2 and 5uM of 5-Aza-2'-deoxycytidine (5-Aza) for 5 days. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-019-1100-4), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: HIF-3 alpha Antibody [NBP1-03155] - Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry-Paraffin: HIF-3 alpha Antibody [NBP1-03155] - Staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

• Reactivity: Mu, Hu(-), Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HIF-3 alpha Antibody - BSA Free Summary

• Immunogen: Synthetic peptide from internal region near aa 575-600 of mouse HIF-3 alpha.
• Specificity: No reactivity is expected against other forms of Hif proteins.
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:14000 - 1:80000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:1000 - 1:8000
• Application Notes: ***Does not work to detect human protein in Western blot.***
• Control:

  NIH 3T3 CoCl2 treated/Untreated Cell Lysate
  L929 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 CoCl2 treated/Untreated Cell Lysate
  Raw 264.7 Hypoxic / Normoxic Cell Lysate
  L929 Hypoxic / Normoxic Cell Lysate
  NIH 3T3 Hypoxic / Normoxic Cell Lysate

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIF-3 alpha Antibody - BSA Free

• Basic-helix-loop-helix-PAS protein MOP7
• BHLHe17
• bHLHe17HIF-3A2
• Class E basic helix-loop-helix protein 17
• HIF-3 alpha
• HIF3A
• HIF-3A4
• HIF-3-alpha
• HIF3-alpha
• hypoxia inducible factor 3, alpha subunit
• hypoxia-inducible factor 3-alpha
• hypoxia-inducible factor-3 alpha 4
• Inhibitory PAS domain protein
• IPAS
• IPASHIF3-alpha-1
• Member of PAS protein 7
• MOP7
• MOP7PAS domain-containing protein 7
• PASD7
• PASD7HIF-3A

Background

One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-3 alpha Antibody (NBP1-03155)(5)

Publications using NBP1-03155

• Ouyang L, Sun Y, Lv D et al. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3 alpha in bone iScience 2022-01-01 [PMID: 35005549] (IHC-P, Mouse)
• Liu FF, Yang H, Li DZ et al. Punicalagin attenuates osteoarthritis progression via regulating Foxo1/Prg4/HIF3 alpha axis Bone 2021-06-22 [PMID: 34171516]
• Romero Y, Castillejos-LOpez M, Romero-GarcIa S et al. Antitumor Therapy under Hypoxic Microenvironment by the Combination of 2-Methoxyestradiol and Sodium Dichloroacetate on Human Non-Small-Cell Lung Cancer Oxidative Medicine and Cellular Longevity 2020-10-12 [PMID: 33149807] (ICC/IF)
• Huang Y, Kapere Ochieng J, Kempen MB et al. Hypoxia Inducible Factor 3alpha Plays a Critical Role in Alveolarization and Distal Epithelial Cell Differentiation during Mouse Lung Development. PLoS One 2013-01-01 [PMID: 23451260] (IHC-P, Mouse)
• Sun Y, Liu X, Zeng X et al. Simvastatin-loaded sulfonated PEEK enhances angiogenesis and osteogenesis via miR-29cb2-mediated HIF-3 alpha downregulation Chemical Engineering Journal 2022-11-01 (IHC, Mouse)

Bioinformatics

• Gene Symbol: HIF3A
• Entrez:
  • 64344(Human)
  • 53417(Mouse)
  • 64345(Rat)
• Uniprot:
  • Q9Y2N7(Human)