HGS Antibody (HL1730) - Azide and BSA Free

Western Blot: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (NBP3-25503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:250. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog pancreas. HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat pancreas. HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (NBP3-25503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ca, Fe
• Applications: WB, ICC/IF, IHC
• Clone: HL1730
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HGS Antibody (HL1730) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HGS Antibody (HL1730) - Azide and BSA Free (NBP3-25503) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human HGS. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HGS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for HGS Antibody (HL1730) - Azide and BSA Free

• FLJ39878
• hepatocyte growth factor-regulated tyrosine kinase substrate
• Hrs
• HRSFLJ11421
• human growth factor-regulated tyrosine kinase substrate
• Protein pp110
• Vps27
• ZFYVE8

Background

HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early end

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGS