Hairless Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) followed by ...read more
Hairless was detected in immersion fixed human NHEK epidermal keratinocytes using 10 µg/mL Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Hairless Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Hairless
Met1-Thr277
Accession # O43593
Specificity
Detects human Hairless in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Positive Control
Hairless Lysate (NBP2-64952)
Publications
Read Publication using
AF5708 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hairless Antibody [Unconjugated]

  • ALUNC
  • AU
  • AUHSA277165
  • FLJ98880
  • hairless (mouse) homolog
  • hairless homolog (mouse)
  • Hairless
  • HR
  • MUHH
  • MUHH1
  • protein hairless

Background

Hairless (HR) is a predicted 145‑155 kDa protein that acts as a corepressor for multiple nuclear receptors. Although it was initially associated with atrichia (hairloss) and WISE gene suppression, it is now known to impact multiple genes in different cell types. In general, it binds both HDACs and select unligated nuclear receptors via multiple domains, thereby repressing gene transcription. Human HR is 1189 amino acids (aa) in length. It contains a C6-type zinc finger DNA binding region (aa 600‑625) and a Jmjc domain (aa 946‑1157) that blocks DNA methylation. The 1189 aa isoform is widely expressed, while a shorter isoform showing a deletion of aa 1072‑1126 is an HR isotype associated with hair follicle Keratin-14+ keratinocytes. An additional isoform shows a 25 aa substitution for aa 1091‑1189. Over
aa 1‑277, human HR shares 82% aa identity with mouse HR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5708
Species: Hu
Applications: WB, ICC
NBP2-29942
Species: Hu, Rt
Applications: WB, Flow, IHC-P
PP-H4537-00
Species: Hu
Applications: WB, IHC, IP, DirELISA
NBP1-69668
Species: Hu
Applications: WB
NBP1-92381
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00009146-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-07087
Species: Hu
NBP1-90044
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89490
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-58824
Species: Hu, Mu
Applications: WB, ChIP, IP, CHIP-SEQ
AF4779
Species: Hu
Applications: WB, IHC
MAB29051
Species: Hu
Applications: IP, CyTOF-ready, ICC, ICFlow
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-66881
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-71770
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-613
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IM, IP
NBP2-01863
Species: Hu
Applications: WB, IHC, IHC-P
AF5708
Species: Hu
Applications: WB, ICC/IF

Publications for Hairless Antibody (AF5708)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Hairless Antibody (AF5708) (0)

There are no reviews for Hairless Antibody (AF5708). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hairless Antibody (AF5708) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Hairless Antibody (AF5708)

Discover related pathways, diseases and genes to Hairless Antibody (AF5708). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Hairless Antibody (AF5708)

Discover more about diseases related to Hairless Antibody (AF5708).
 

Pathways for Hairless Antibody (AF5708)

View related products by pathway.

PTMs for Hairless Antibody (AF5708)

Learn more about PTMs related to Hairless Antibody (AF5708).

Blogs on Hairless

There are no specific blogs for Hairless, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hairless Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol HR
Entrez
Uniprot