BAM1676) to detect phospho-HGF R (p-HGF R). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_lightbox"> BAM1676) to detect phospho-HGF R (p-HGF R). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." alt="The human epidermoid carcinoma cell line, A431, was treated with 100 ng/mL recombinant human HGF for five minutes to induce tyrosine phosphorylation of HGF R. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-HGF R polyclonal antibody and Protein G agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-HGF R (p-HGF R). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_thumb" />

BAF358). " class="big_lightbox"> BAF358). " class="big_thumb" />

358-MT), human EGF R (Catalog #1095-ER) or human IGF-I sR (Catalog #305-GR) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant HGF R. " class="big_lightbox"> 358-MT), human EGF R (Catalog #1095-ER) or human IGF-I sR (Catalog #305-GR) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant HGF R. " class="big_thumb" />