Recombinant Human HGF Protein

Product Discontinued

Recombinant Human HGF Protein Summary

• Description: Recombinant bioactive protein for Human HGF
  
  

  Source:CHO cells
  Amino Acid Sequence:( P14210)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: HGF
• Purity: >97%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 80.017 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Lyophilized from a 1 mg/ml solution containing PBS (pH 7.4), 0.05% Tween 20, 100 mM L-arginine.
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile MilliQ (R) H2O to no less than 100 ug/ml, which can then be further diluted into other aqueous solutions.

Alternate Names for Recombinant Human HGF Protein

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.HGF Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting analpha-chain of 463 amino acids and Beta -chain of 234 having a total molecular mass of 80.017 kDa. The HGF is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HGF