Mouse HGF ELISA Kit (Colorimetric) Summary
Description |
Quality control test: Standard Curve. Hgf (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Hgf. |
Sensitivity |
100 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 12% |
Intra-Assay |
CV% < 10% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
HGF |
Applications/Dilutions
Dilutions |
|
Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
Components
|
- Assay Diluent (Item E): 5x concentrated buffer. For Detection Antibody(Item F) and HRP-Streptavidin concentrate (Item G) diluent
- Cell lysate buffer (Item J): 2x cell lysate buffer
- Detection Antibody HGF (Item F): biotinylated anti-mouse HGF (each vialis enough to assay half microplate)
- HGF Microplate (Item A): coated with anti-mouse HGF
- HRP-Streptavidin concentrates (Item G): 500x concentratedHRP-conjugated Streptavidin
- Sample Diluent Buffer (Item D): 5x concentrated buffer. ForStandard/Sample (cell lysate/tissue lysate) diluent
- Standards (Item C): recombinant mouse HGF
- Stop Solution (Item I): 0.2 M sulfuric acid
- TMB One-Step Substrate Reagent (Item H): of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse HGF ELISA Kit (Colorimetric)
Background
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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