HGF Antibody (24612.111)

Product Discontinued

HGF Antibody (24612.111) Summary

• Immunogen: Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
• Localization: Secreted
• Specificity: The product shows no cross reactivity with other tested cytokines using indirect ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HGF
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-1 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: detects <1.2 ng/well of rhHGF. WB: Use under non-reducing conditions. Predicted molecular weight: 83.1 kDa. Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22C.
• Control:

  HGF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for HGF Antibody (24612.111)

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGF
• Entrez:
  • 3082(Human)