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HGF Antibody (24516) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
24516
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

HGF Antibody (24516) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Specificity
Detects recombinant human (rh)HGF in direct ELISAs and Western blots. In direct ELISAs, less than 0.25% cross-reactivity was observed with rhMSP.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HGF
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human HGF Biotinylated Antibody (Catalog number BAF294)
Standard: Recombinant Human HGF (Catalog number 294-HG)
Publications
Read Publications using
MAB694 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Antibody (24516) [Unconjugated]

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGF Antibody (MAB694)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA Development (Capture), ELISA Microarray Development, Flow Cytometry, ICC, Western Blot.


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ELISA Development (Capture)
(1)
ELISA Microarray Development
(1)
Flow Cytometry
(1)
ICC
(1)
Western Blot
(1)
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Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB694 Applications Species
Shayne Lavondua Ford, Terkild Brink Buus, Claudia Nastasi, Carsten Geisler, Charlotte Menné Bonefeld, Niels Ødum et al. In vitro differentiated human CD4+ T cells produce hepatocyte growth factor Frontiers in Immunology 7/13/2023 [PMID: 37520551]
Shivaani Kummar, Apurva K. Srivastava, Tony Navas, Fabiola Cecchi, Young H. Lee, Donald P. Bottaro et al. Combination therapy with pazopanib and tivantinib modulates VEGF and c-MET levels in refractory advanced solid tumors Investigational New Drugs 12/1/2021 [PMID: 34180036]
T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells PLoS ONE, 2018-06-21;13(6):e0199361. 2018-06-21 [PMID: 29928062] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Tatiana S. Zaitseva, Cynthia Alcazar, Maedeh Zamani, Luqia Hou, Steve Sawamura, Eduard Yakubov et al. Aligned Nanofibrillar Scaffolds for Controlled Delivery of Modified mRNA Tissue Engineering Part A 1/1/2019 [PMID: 29717619]
Yasuhiro Watanabe, Yasuhiro Kazuki, Kanako Kazuki, Mitsutaka Ebiki, Mami Nakanishi, Kazuomi Nakamura et al. Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis Molecular Therapy - Nucleic Acids 10/6/2015 [PMID: 26440597]
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human) ELISA Microarray Development Human
Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome. J Trauma, 2004-02-01;56(2):259-64. 2004-02-01 [PMID: 14960965] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HGF