HGF Antibody (24516) [Unconjugated]

294-HG) was serially diluted and captured by Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB694) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 294-HG) was serially diluted and captured by Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB694) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human HGF (Catalog # 294-HG) was serially diluted and captured by Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB694) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human HGF (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 24516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HGF Antibody (24516) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
  Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
• Specificity: Detects recombinant human (rh)HGF in direct ELISAs and Western blots. In direct ELISAs, less than 0.25% cross-reactivity was observed with rhMSP.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HGF
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human HGF Biotinylated Antibody (Catalog number BAF294)
  Standard: Recombinant Human HGF (Catalog number 294-HG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Antibody (24516) [Unconjugated]

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HGF Antibody (MAB694)(7)

Publications using MAB694

• Shayne Lavondua Ford, Terkild Brink Buus, Claudia Nastasi, Carsten Geisler, Charlotte Menné Bonefeld, Niels Ødum et al. In vitro differentiated human CD4+ T cells produce hepatocyte growth factor Frontiers in Immunology 7/13/2023 [PMID: 37520551]
• Shivaani Kummar, Apurva K. Srivastava, Tony Navas, Fabiola Cecchi, Young H. Lee, Donald P. Bottaro et al. Combination therapy with pazopanib and tivantinib modulates VEGF and c-MET levels in refractory advanced solid tumors Investigational New Drugs 12/1/2021 [PMID: 34180036]
• T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells PLoS ONE, 2018-06-21;13(6):e0199361. 2018-06-21 [PMID: 29928062] (ELISA Development (Capture), Mouse)
• Tatiana S. Zaitseva, Cynthia Alcazar, Maedeh Zamani, Luqia Hou, Steve Sawamura, Eduard Yakubov et al. Aligned Nanofibrillar Scaffolds for Controlled Delivery of Modified mRNA Tissue Engineering Part A 1/1/2019 [PMID: 29717619]
• Yasuhiro Watanabe, Yasuhiro Kazuki, Kanako Kazuki, Mitsutaka Ebiki, Mami Nakanishi, Kazuomi Nakamura et al. Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis Molecular Therapy - Nucleic Acids 10/6/2015 [PMID: 26440597]
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome. J Trauma, 2004-02-01;56(2):259-64. 2004-02-01 [PMID: 14960965] (Flow Cytometry, ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: HGF