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Hexokinase 1 Antibody

Images

 
Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample (30 ug of whole cell lysate)C: zebrafish muscle7.5% SDS PAGEdiluted at 1:1000
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody [NBP1-31600] - Analysis of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin ...read more
Immunohistochemistry Whole-Mount: Hexokinase 1 Antibody [NBP1-31600] - Hexokinase 1 antibody detects Hexokinase 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization ...read more
Western Blot: Hexokinase 1 Antibody [NBP1-31600] - Sample(30 ug whole cell lysate)A:A431 B:H1299 C:HeLa S3 D:Hep G2 7.5% SDS PAGE, antibody diluted at 1:3000.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Human breast cancer, using HXK Iantibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody [NBP1-31600] - Analysis of paraffin-embedded zebrafish tissue, using Hexokinase 1 antibody at 1:300 dilution.

Product Details

Summary
Reactivity Hu, Bv, Ze, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Hexokinase 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Hexokinase 1. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane
Predicted Species
Mouse (94%), Rat (92%), Porcine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
IHC-WM-Assay dependent
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31600 in the following applications:

  • WB
    1 publication

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 31261667). Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%), Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Hexokinase 1 Antibody

  • Brain form hexokinase
  • EC 2.7.1
  • EC 2.7.1.1
  • glycolytic enzyme
  • Hexokinase 1
  • Hexokinase type I
  • hexokinase-1
  • HK I
  • HK1
  • HK1-ta
  • HK1-tb
  • HK1-tc
  • HKI
  • HXK1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Hexokinase 1 Antibody (NBP1-31600)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HK1