Hemoglobin A1 Antibody (SN70-09)

Western Blot: Hemoglobin A1 Antibody (SN70-09) [NBP2-67716] - Western blot analysis of Hemoglobin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Immunohistochemistry-Paraffin: Hemoglobin A1 Antibody (SN70-09) [NBP2-67716] - Analysis of paraffin-embedded human spleen tissue using anti-Hemoglobin subunit alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Hemoglobin A1 Antibody (SN70-09) [NBP2-67716] - Analysis of paraffin-embedded human lung tissue using anti-Hemoglobin subunit alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Hemoglobin A1 Antibody (SN70-09) [NBP2-67716] - Analysis of paraffin-embedded mouse embryo tissue using anti-Hemoglobin subunit alpha antibody. Counter stained with hematoxylin.

Product Discontinued

Hemoglobin A1 Antibody (SN70-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Hemoglobin A1 aa 71-120 / 142. (SwissProt: P69905 Human; SwissProt: P01942 Mouse; SwissProt: P01946 Rat)
• Localization: Cytosol, membrane, extracellular region
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HBA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Hemoglobin A1 Antibody (SN70-09)

• alpha one globin
• alpha-1 globin
• alpha-1-globin
• alpha-globin
• CD31
• hemoglobin alpha 1 globin chain
• Hemoglobin alpha chain
• hemoglobin alpha-1 chain
• hemoglobin subunit alpha
• hemoglobin, alpha 1
• MGC126895
• MGC126897

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hemoglobin A1 Antibody (NBP2-67716)(1)

Publication using NBP2-67716

• Wenzek C, Steinbach P, WirsdOrfer F et al. CD47 restricts antiviral function of alveolar macrophages during influenza virus infection iScience 2022-12-22 [PMID: 36437873] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: HBA1