Hemoglobin A1 Antibody (4F9) - Azide and BSA Free

Western Blot: Hemoglobin A1 Antibody (4F9) [H00003039-M02] - HBA1 monoclonal antibody (M02), clone 4F9. Analysis of HBA1 expression in human placenta.

Sandwich ELISA: Hemoglobin A1 Antibody (4F9) [H00003039-M02] - Detection limit for recombinant GST tagged HBA1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hemoglobin A1 Antibody (4F9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HBA1 (NP_000549, 33 a.a. ~ 142 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR
• Specificity: HBA1 (4F9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HBA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hemoglobin A1 Antibody (4F9) - Azide and BSA Free

• alpha one globin
• alpha-1 globin
• alpha-1-globin
• alpha-globin
• CD31
• hemoglobin alpha 1 globin chain
• Hemoglobin alpha chain
• hemoglobin alpha-1 chain
• hemoglobin subunit alpha
• hemoglobin, alpha 1
• MGC126895
• MGC126897

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hemoglobin A1 Antibody (H00003039-M02)(1)

Publication using H00003039-M02

• Aoyama S, Nakano H, Danbara M et al. The differentiating and apoptotic effects of 2-aza-5'-deoxycytidine are dependent on the PU.1 expression level in PU.1-transgenic K562 cells. Biochem Biophys Res Commun. 2012-03-20 [PMID: 22459451]

Bioinformatics

• Gene Symbol: HBA1
• Entrez:
  • 3039(Human)
• OMIM:
  • 141800(Human)
• Uniprot:
  • NP_000549(Human)