HDJ2 Antibody - Azide and BSA Free

Western Blot: HDJ2 Antibody - Azide and BSA Free [HDJ2] - Western blot analysis of lysates from wild type(WT) and HDJ2 knockout (KO) 293T(KO) cells, using [KO Validated] HDJ2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: HDJ2 Antibody - Azide and BSA Free [HDJ2] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] HDJ2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HDJ2 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human HDJ2 (NP_001530.1). MECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for HDJ2 Antibody - Azide and BSA Free

• dj-2
• DjA1
• DnaJ (Hsp40) homolog, subfamily A, member 1
• dnaJ homolog subfamily A member 1
• DnaJ protein homolog 2
• DNAJ2
• HDJ2
• hDj-2
• Heat shock 40 kDa protein 4
• Heat shock protein J2
• heat shock protein, DNAJ-like 2
• HSDJ
• HSJ2
• HSJ-2
• HSPF4hdj-2
• Human DnaJ protein 2
• NEDD7
• neural precursor cell expressed, developmentally down-regulated 7

Background

HDJ 2 is a novel human homolog of E. coli's DNA J protein from heat shock protein 40 family. Hsp 40 is involved in chaperonic protein folding. HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the golgi complex, nucleous and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: DNAJA1