HDAC3 Antibody - BSA Free

Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126] - Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by ExtrAvidin(R)-Peroxidase

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 12.5 mg/ml

HDAC3 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of human and chicken HDAC3.
• Epitope: The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
• Localization: Nuclear
• Specificity: Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kD).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:2500
  • Microarray
  • Western Blot 1:2000-1:10000
• Application Notes: An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate
  Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 12.5 mg/ml
• Purity: IgG purified

Alternate Names for HDAC3 Antibody - BSA Free

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC3 Antibody (NB500-126)(9)

Publications using NB500-126

• Wang F, Gao Y, Xue S et al. SCARB2 drives hepatocellular carcinoma tumor initiating cells via enhanced MYC transcriptional activity Nat Commun 2023-09-22 [PMID: 37739936]
• Hern�ndez-Carralero E, Cabrera E, Rodr�guez-Torres G et al. ATXN3 controls DNA replication and transcription by regulating chromatin structure Nucleic acids research 2023-03-27 [PMID: 36971114] (ICC/IF, Human)
• Tanaka H, Muto A, Shima H et al. Epigenetic Regulation of the Blimp-1 Gene in B Cells Involves Bach2 and Histone Deacetylase 3 J. Biol. Chem. 2016-01-19 [PMID: 26786103] (IP, Mouse)
• Furuya, F, C J Guigon et al. Nuclear Receptor Corepressor Is a Novel Regulator of Phosphatidylinositol 3-Kinase Signaling. 27: 6116-6126. 2007-01-01 [PMID: 17606624]
• Li J et al. Expression functional characterization of recombinant human HDAC1 HDAC3. Life Sci 74:2693-705. 2004-01-01 [PMID: 15043985]
• Yang SH. Sharrocks AD SUMO promotes HDAC-mediated transcriptional repression. Mol Cell 13:611-7. 2004-01-01 [PMID: 14992729]
• Glaser KB et al. Role of class I class II histone deacetylases in carcinoma cells using siRNA. Biochem Biophys Res Commun 310:529-36. 2003-01-01 [PMID: 14521942]
• Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73. 1999-01-01 [PMID: 10220385]
• Hassig CA et al. A role for histone deacetylase activity in HDAC1-mediated transcriptional repression. Proc Natl Acad Sci U S A 95:3519-24. 1998-01-01 [PMID: 9520398]

Bioinformatics

• Gene Symbol: HDAC3
• Entrez:
  • 8841(Human)
  • 15183(Mouse)
  • 84578(Rat)
• Uniprot:
  • O15379(Human)