HDAC3 Antibody

Images

 
Western Blot: HDAC3 Antibody [NB500-126] - Analysis of HDAC3 Antibody [NB500-126]-Monoclonal Anti-HDAC3 antibody produced in mouse clone 3E11, purified immunoglobulin, buffered aqueous solution
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone, stained with HDAC3 Antibody diluted at 1:200.
Western Blot: HDAC3 Antibody [NB500-126] - Cell line lysates (Lanes1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK) were separated on SDS-PAGE and probed with 1:5,000 Rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, MiAr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
12.5 mg/ml

Order Details

HDAC3 Antibody Summary

Immunogen
Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of human and chicken HDAC3.
Epitope
The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
Localization
Nuclear
Specificity
Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kD).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:2500
  • Microarray
Application Notes
IHC-P: Use human lymph node sections. WB: Use nuclear HeLa human epitheloid carcinoma cells. Predicted molecular weight: 48.8 kDa.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB500-126 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
12.5 mg/ml
Purity
IgG purified

Alternate Names for HDAC3 Antibody

  • EC 3.5.1.98
  • HD3RPD3-2RPD3
  • HDAC3
  • Histone Deacetylase 3
  • KDAC3
  • RPD3-2
  • SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC3 Antibody (NB500-126)(7)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HDAC3
Entrez
Uniprot