HDAC3 Overexpression Lysate (Native) Summary
Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC200605 and based on accession number NM_003883. The protein contains a C-terminal DDK tag. |
Specificity |
Homo sapiens histone deacetylase 3 (HDAC3), mRNA. |
Gene |
HDAC3 |
Applications/Dilutions
Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug). |
Theoretical MW |
48.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles. |
Buffer |
RIPA buffer |
Lysate Details for HDAC3
Type |
Overexpression |
Protein State |
Native |
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for HDAC3 Overexpression Lysate (Native)
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ChIP, IP, PLA, CHIP-SEQ
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP, ICC
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
Species: Hu, Mu
Applications: WB, ChIP, IP
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Hu
Applications: ELISA, ICC/IF
Species: Hu, Mu, Rt, Ma
Applications: WB, ChIP, DB, ICC/IF
Species: Hu
Applications: WB
Publications for HDAC3 Lysate (NBL1-11481) (0)
There are no publications for HDAC3 Lysate (NBL1-11481).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for HDAC3 Lysate (NBL1-11481) (0)
There are no reviews for HDAC3 Lysate (NBL1-11481).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for HDAC3 Lysate (NBL1-11481) (0)
Additional HDAC3 Products
Bioinformatics Tool for HDAC3 Lysate (NBL1-11481)
Discover related pathways, diseases and genes to HDAC3 Lysate (NBL1-11481). Need help?
Read the
Bioinformatics Tool Guide for instructions on using this tool.
Diseases for HDAC3 Lysate (NBL1-11481)
Discover more about diseases related to HDAC3 Lysate (NBL1-11481).
| | Pathways for HDAC3 Lysate (NBL1-11481)
View related products by pathway.
|
PTMs for HDAC3 Lysate (NBL1-11481)
Learn more about PTMs related to HDAC3 Lysate (NBL1-11481).
| | Research Areas for HDAC3 Lysate (NBL1-11481)
Find related products by research area.
|
Blogs on HDAC3