HDAC3 Overexpression Lysate (Native)

Western Blot: HDAC3 Overexpression Lysate (Adult Normal) [NBL1-11481] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HDAC3.

• Reactivity: Hu
• Applications: WB

HDAC3 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC200605 and based on accession number NM_003883. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens histone deacetylase 3 (HDAC3), mRNA.
• Gene: HDAC3

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 48.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HDAC3 Overexpression Lysate (Native)

• EC 3.5.1.98
• HD3RPD3-2RPD3
• histone deacetylase 3
• SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3