HCLS1 Antibody - BSA Free

Western Blot: HCLS1 Antibody [NBP2-16787] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: HCLS1 Antibody [NBP2-16787] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 ug of HCLS1 antibody 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HCLS1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HCLS1. The exact sequence is proprietary.
• Localization: Membrane; Peripheral membrane protein (Probable); Cytoplasm (Probable); Mitochondrion
• Predicted Species: Mouse (95%), Rat (95%), Rhesus Macaque (98%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HCLS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HCLS1 Antibody - BSA Free

• Cortactin-like
• CTTNL
• HCLS1
• hematopoietic cell-specific Lyn substrate 1hematopoietic lineage cell-specific protein
• HS1lckBP1
• LckBP1
• p75

Background

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HCLS1
• Uniprot:
  • P14317()