Mouse HB-EGF ELISA Kit (Colorimetric)

ELISA: Mouse HB-EGF ELISA Kit (Colorimetric) [NBP2-62763] - Samples were spiked with high concentrations of Mouse HB-EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse HB-EGF ELISA Kit (Colorimetric) [NBP2-62763] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.625 - 1000 pg/mL
• Sensitivity: 9.375 pg/mL

Mouse HB-EGF ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed.
• Standard Curve Range: 15.625 - 1000 pg/mL
• Sensitivity: 9.375 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 4.95
• Intra-Assay: %CV < 5.17
• Spike Recovery: 88 - 105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HBEGF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse HB-EGF ELISA Kit (Colorimetric)

• diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
• Dtr
• DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
• Dts
• DTSF
• HBEGF
• HB-EGF
• Hegfl
• heparin-binding EGF-like growth factor
• heparin-binding epidermal growth factor
• proheparin-binding EGF-like growth factor

Background

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for HB-EGF ELISA Kit (NBP2-62763). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HBEGF