HB-EGF Antibody [Unconjugated]

Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND50 is typically 0.1-0.6 µg/mL.

HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dynamic expression and processing of Tgf-alpha and Hb-egf during multistep pancreatic neuroendocrine tumorigenesis (PNET). (A) Relative expression of Egf family members in cDNAs prepared from total RNA extracts of successive stages of neoplastic progression (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of the various forms of Tgf-alpha polypeptides in total protein extracts from successive stages of disease progression. (C) Analogous Western blot analysis of the various forms of Hb-egf polypeptides in total protein extracts from the successive disease stages. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

BHLHE40-knockdown reduced hypoxia-induced expression of a panel of cytokines and growth factors. a Heatmaps of cytokines and growth factors whose hypoxia-induced expression (1% O2 at 6 h or 48 h, fold-change ≥ 1.5 in two independent experiments) was diminished by BHLHE40-knockdown (KD) in LM cells. The gene expression levels were determined using the Illumina Human HT-12 expression BeadChips. Normalized (quantile normalization) hybridization signals were log2 transformed and standardized by genes across experiment conditions to generate the heatmap. b Heatmaps of a subset of genes list in a whose expression was affected by BHLHE40-KD in LM cells exposed to hypoxia combined with low (1 mM) glucose (1%O2/LG, 4 h). The gene expression levels were determined using the Affymetrix Human Gene 1.0 ST array. c Heatmaps of hypoxia-induced genes whose expression was not significantly affected by BHLHE40-KD in LM cells as determined by the Illumina Human HT-12 expression BeadChips. d Expression of luciferase reporters driven by hypoxia-responsive elements of ITGA6 or LDHA was not affected by BHLHE40 knockout (KO) by CRISPR/Cas9 editing in MDA-MB-231 cells, in the absence or presence of exogenous HIF1A. Luciferase activities were normalized to co-transfected CMV-beta -galactosidase and presented as mean ± SD (n = 6). e Expression of genes in control LM empty vector (EV) and LM BHLHE40-KD cells exposed to 1%O2/LG (4 h). mRNA expression levels were determined by qPCR, normalized to RPL13A, and presented as mean ± SD (n = 6). *p < 0.05 (n = 6, 1%O2/LG vs. untreated control), **p < 0.05 (n = 6, KD vs. EV), one-way ANOVA followed by Tukey’s post-hoc tests. f mRNA and protein expression levels of HBEGF and CTGF in primary xenograft tumors, determined by qPCR and immunoblotting, respectively. *p < 0.05 (n = 6, KD vs. EV), Student’s t test. Representative immunoblotting images of three tumors of KD or EV cells are presented Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30285805), licensed under a CC-BY license. Not internally tested by R&D Systems.

Egfr activity contributes to RT2 tumor growth and angiogenic switching. (A) Relative expression quantified by real-time quantitative PCR of Erbb family members in cDNAs derived from total RNA extracts of successive stages of pancreatic neuroendocrine tumorigenesis from RIP1-Tag2 (RT2) transgenic mice (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of protein extracts from successive stages of RT2-derived lesions. (C) Western blot analysis of protein extracts from RT2-derived tumors 4 h after treatment with a vehicle solution or erlotinib (80 mg/kg). (D-F) Comparison of the average tumor burden of RT2 mice treated daily with a control solution (vehicle) or with (D) gefitinib (80 mg/kg), (E) CI-1033 (80 mg/kg), both from 11.5 to 14.5 weeks of age, or with (F) erlotinib (80 mg/kg), from 12 to 16 weeks of age with an additional vehicle-treated time point at 14 weeks. (G) Comparison of the average number of hemorrhagic angiogenic islets per pancreas of RT2 mice treated daily with a vehicle solution or with erlotinib (80 mg/kg) or CI-1033 (80 mg/kg) from 6 to 9 weeks of age. (N = number of animals per treatment group). *P < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hb-egf contributes to the angiogenic switch and neovascularization of PNET tumors. (A) Comparison of the average number of hemorrhagic angiogenic islets in wild-type (wt) and Hb-egf mutant (Hb) 9-week-old RT2 mice. (B-C) Comparison of the average (B) tumor burden or (C) tumor number in wild-type and Hb-egf mutant 14-week-old RT2 mice. (D) Western blot analysis of Hb-egf protein expression in total protein extracts from pools of 5 wild-type or 5 Hb-egf mutant tumors. (E-F) Average percentage of (E) dividing tumor cells (phospho-histone H3 positive) or (F) apoptotic cells (TdT-mediated dUTP-biotin nick end-labeling [TUNEL] positive) in wild-type or Hb-egf mutant tumors at 14 weeks of age. (G) Average vessel density (ratio of Meca-32 stained area to total section area) in wild-type or Hb-egf mutant tumors at 14 weeks of age. (H-I) Representative micrographs of (H) wild-type or (I) Hb-egf mutant RT2 tumors stained with Meca-32 (red) and DAPI (blue) (200x); panels are representative of 20 wild-type and 18 Hb-egf RT2 tumors dissected from at least 5 independent mice of each genotype. (N = number of independent mice [A-C] or tumor fields [E-G] analyzed per genotype; 1-2 fields per tumor.) *P < 0.001. **P < 0.01. ***P < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HB-EGF Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE)
  Asp63-Leu148
  Accession # Q99075
• Specificity: Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HBEGF
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.6 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human HB-EGF Antibody (Catalog # AF-259-NA) ELISA Detection:Human HB-EGF Biotinylated Antibody (Catalog # BAF259) Standard: Recombinant Human HB-EGF (Catalog # 259-HE)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HB-EGF Antibody [Unconjugated]

• diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
• Dtr
• DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
• Dts
• DTSF
• HBEGF
• HB-EGF
• Hegfl
• heparin-binding EGF-like growth factor
• heparin-binding epidermal growth factor
• proheparin-binding EGF-like growth factor

Background

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O-glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis. HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the Her2/ErbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha , and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HB-EGF Antibody (AF-259-NA)(61)

Publications using AF-259-NA

• Kuehl, M;Okabayashi, Y;Wong, MN;Gernhold, L;Gut, G;Kaiser, N;Schwerk, M;Gräfe, SK;Ma, FY;Tanevski, J;Schäfer, PSL;Mezher, S;Sarabia Del Castillo, J;Goldbeck-Strieder, T;Zolotareva, O;Hartung, M;Delgado Chaves, FM;Klinkert, L;Gnirck, AC;Spehr, M;Fleck, D;Joodaki, M;Parra, V;Shaigan, M;Diebold, M;Prinz, M;Kranz, J;Kux, JM;Braun, F;Kretz, O;Wu, H;Grahammer, F;Heins, S;Zimmermann, M;Haas, F;Kylies, D;Wanner, N;Czogalla, J;Dumoulin, B;Zolotarev, N;Lindenmeyer, M;Karlson, P;Nyengaard, JR;Sebode, M;Weidemann, S;Wiech, T;Groene, HJ;Tomas, NM;Meyer-Schwesinger, C;Kuppe, C;Kramann, R;Karras, A;Bruneval, P;Tharaux, PL;Pastene, D;Yard, B;Schaub, JA;McCown, PJ;Pyle, L;Choi, YJ;Yokoo, T;Baumbach, J;Sáez, PJ;Costa, I;Turner, JE;Hodgin, JB;Saez-Rodriguez, J;Huber, TB;Bjornstad, P;Kretzler, M;Lenoir, O;Nikolic-Paterson, DJ;Pelkmans, L;Bonn, S;Puelles, VG; Pathology-oriented multiplexing enables integrative disease mapping Nature 2025-07-18 [PMID: 40681898] (IF/IHC, Human)
• Won, KJ;Lee, R;Choi, SH;Kim, JH;Hwang, SH;Nah, SY; Gintonin-Induced Wound-Healing-Related Responses Involve Epidermal-Growth-Factor-like Effects in Keratinocytes International journal of molecular sciences 2023-09-14 [PMID: 37762395] (Western Blot, Human)
• Okamoto, M;Sasai, M;Kuratani, A;Okuzaki, D;Arai, M;Wing, JB;Sakaguchi, S;Yamamoto, M; A genetic method specifically delineates Th1-type Treg cells and their roles in tumor immunity Cell reports 2023-07-25 [PMID: 37440410] (IHC, Mouse)
• Rafael Soares Godoy, Nicholas D Cober, David P Cook, Emma McCourt, Yupu Deng, Liyuan Wang et al. Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation eLife 2023-04-20 [PMID: 37078698] (Immunohistochemistry, Transgenic Mouse)
• Rafael Soares Godoy, Nicholas D Cober, David P Cook, Emma McCourt, Yupu Deng, Liyuan Wang et al. Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation eLife 4/20/2023 [PMID: 37078698]
• Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis Nature Communications, 2023-03-22;14(1):1588. 2023-03-22 [PMID: 36949050] (IHC, Mouse)
• Nancy G. Casanova, Vivian Reyes-Hernon, Taylor Gregory, Belinda Sun, Tadeo Bermudez, Matthew K. Hufford et al. Biochemical and genomic identification of novel biomarkers in progressive sarcoidosis: HBEGF, eNAMPT, and ANG-2 Frontiers in Medicine 10/25/2022 [PMID: 36388923]
• C Zhang, LK Vincelette, F Reimann, SD Liberles A brainstem circuit for nausea suppression Cell Reports, 2022-06-14;39(11):110953. 2022-06-14 [PMID: 35705049] (IHC, Mouse)
• Haixiao Wang, Lingjuan He, Yan Li, Wenjuan Pu, Shaohua Zhang, Ximeng Han et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo Journal of Biological Chemistry 6/1/2022 [PMID: 35461809]
• Barbara Schreier, Virginie Dubourg, Stefanie Hübschmann, Sindy Rabe, Sigrid Mildenberger, Michael Gekle Synergy of epidermal growth factor (EGFR) and angiotensin II (AT1R) receptor determines composition and temporal pattern of transcriptome variation Cellular and Molecular Life Sciences 1/1/2022 [PMID: 34921637]
• Jun Watanabe, Yuki Takayanagi, Masahide Yoshida, Tatsuya Hattori, Michiko Saito, Kenji Kohno et al. Conditional ablation of vasopressin‐synthesizing neurons in transgenic rats Journal of Neuroendocrinology 12/1/2021 [PMID: 34748241]
• M Fujita, M Sasada, M Eguchi, T Iyoda, S Okuyama, T Osawa, K Tsuzuranuk, M Sakamoto, Y Hagihara, M Matsumura, S Osada, H Kodama, Y Higami, F Fukai Induction of cellular senescence in fibroblasts through beta1-integrin activation by tenascin-C-derived peptide and its protumor effect American journal of cancer research, 2021-09-15;11(9):4364-4379. 2021-09-15 [PMID: 34659892] (Neutralization, Human)
• CK Stubbs, M Biancucci, V Vidimar, KJF Satchell RAS specific protease induces irreversible growth arrest via p27 in several KRAS mutant colorectal cancer cell lines Scientific Reports, 2021-09-09;11(1):17925. 2021-09-09 [PMID: 34504197] (Western Blot, Human)
• G Hoeffel, G Debroas, A Roger, R Rossignol, J Gouilly, C Laprie, L Chasson, PV Barbon, A Balsamo, A Reynders, A Moqrich, S Ugolini Sensory�neuron-derived TAFA4 promotes macrophage tissue repair functions Nature, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012116] (Flow Cytometry, Mouse)
• Y Chen, K Xu, L Piccoli, M Foglierini, J Tan, W Jin, J Gorman, Y Tsybovsky, B Zhang, B Traore, C Silacci-Fr, C Daubenberg, PD Crompton, R Geiger, F Sallusto, PD Kwong, A Lanzavecch Structural basis of malaria RIFIN binding by LILRB1-containing antibodies Nature, 2021-03-31;0(0):. 2021-03-31 [PMID: 33790470] (ELISA Capture, Human)
• Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno et al. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer Cell Death & Differentiation 5/1/2021 [PMID: 33303976]
• Chuchu Zhang, Judith A. Kaye, Zerong Cai, Yandan Wang, Sara L. Prescott, Stephen D. Liberles Area Postrema Cell Types that Mediate Nausea-Associated Behaviors Neuron 2/1/2021 [PMID: 33278342]
• Szu-Wei Lee, Cosimo Commisso Metabolic regulation of EGFR effector and feedback signaling in pancreatic cancer cells requires K-Ras Biochemical and Biophysical Research Communications 12/1/2020 [PMID: 32972751]
• RL Hastings, M Mikesh, YI Lee, WJ Thompson Morphological remodeling during recovery of the neuromuscular junction from terminal Schwann cell ablation in adult mice Sci Rep, 2020-07-07;10(1):11132. 2020-07-07 [PMID: 32636481] (IHC, Mouse)
• E Fu, T Liu, S Yu, X Chen, L Song, H Lou, F Ma, S Zhang, S Hussain, J Guo, J Sun, P Yu, X Mao, L Wei M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB?EGF Oncol. Rep., 2020-05-29;44(2):698-710. 2020-05-29 [PMID: 32627036] (Cell Culture, Human)
• Sara L. Prescott, Benjamin D. Umans, Erika K. Williams, Rachael D. Brust, Stephen D. Liberles An Airway Protection Program Revealed by Sweeping Genetic Control of Vagal Afferents Cell 4/1/2020 [PMID: 32259485]
• L Rao, D Giannico, P Leone, AG Solimando, E Maiorano, C Caporusso, L Duda, R Tamma, R Mallamaci, N Susca, A Buonavogli, MC Da Vià, D Ribatti, V De Re, A Vacca, V Racanelli HB-EGF-EGFR Signaling in Bone Marrow Endothelial Cells Mediates Angiogenesis Associated with Multiple Myeloma Cancers (Basel), 2020-01-10;12(1):. 2020-01-10 [PMID: 31936715] (Neutralization, Human)
• Vanessa M. Puñal, Caitlin E. Paisley, Federica S. Brecha, Monica A. Lee, Robin M. Perelli, Jingjing Wang et al. Large-scale death of retinal astrocytes during normal development is non-apoptotic and implemented by microglia PLOS Biology 10/18/2019 [PMID: 31626642]
• Vanessa M. Puñal, Caitlin E. Paisley, Federica S. Brecha, Monica A. Lee, Robin M. Perelli, Jingjing Wang et al. Large-scale death of retinal astrocytes during normal development is non-apoptotic and implemented by microglia PLOS Biology 2019-10-18 [PMID: 31626642] (Mouse)
• NM Miranda da, CT Saab Abe, GP Mitre, RA Mesquita, MSD Silva Kata, AL Ribeiro Ri, RG Jaeger, SM Alves-Júni, AM Smith, JJV Pinheiro HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways Cells, 2019-07-17;8(7):. 2019-07-17 [PMID: 31319505] (IHC-P, Human)
• S Grainger, N Nguyen, J Richter, J Setayesh, B Lonquich, CH Oon, JM Wozniak, R Barahona, CN Kamei, J Houston, M Carrillo-T, IA Drummond, D Gonzalez, K Willert, D Traver EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells Nat. Cell Biol., 2019-05-20;21(6):721-730. 2019-05-20 [PMID: 31110287] (Neutralization, Human)
• DC Branco, NMM da Costa, CTS Abe, MSDS Kataoka, JJV Pinheiro, SM Alves Júni HIF-1&amp;alpha, NOTCH1, ADAM12, and HB-EGF are overexpressed in mucoepidermoid carcinoma Oral Surg Oral Med Oral Pathol Oral Radiol, 2018-10-05;127(1):e8-e17. 2018-10-05 [PMID: 30415904] (IHC, Human)
• Cheng-Chiu Huang, Weishan Yang, Changxiong Guo, Haowu Jiang, Fengxian Li, Maolei Xiao et al. Anatomical and functional dichotomy of ocular itch and pain Nature Medicine 8/1/2018 [PMID: 29988128]
• Rahul Dhandapani, Cynthia Mary Arokiaraj, Francisco J. Taberner, Paola Pacifico, Sruthi Raja, Linda Nocchi et al. Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons Nature Communications 4/24/2018 [PMID: 29691410]
• Rahul Dhandapani, Cynthia Mary Arokiaraj, Francisco J. Taberner, Paola Pacifico, Sruthi Raja, Linda Nocchi et al. Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons Nature Communications 2018-04-24 [PMID: 29691410] (Immunohistochemistry, Human)
• M Stevens, CR Neal, AHJ Salmon, DO Bates, SJ Harper, S Oltean Vascular Endothelial Growth Factor-A165b Restores Normal Glomerular Water Permeability in a Diphtheria-Toxin Mouse Model of Glomerular Injury Nephron, 2018-01-26;0(0):. 2018-01-26 [PMID: 29393270] (IHC-P, Mouse)
• Alberto Roselló-Díez, Daniel Stephen, Alexandra L Joyner Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth eLife 7/25/2017 [PMID: 28741471]
• Marlies Schrevel, E. Michelle Osse, Frans A. Prins, J. Baptist M.Z. Trimbos, Gert Jan Fleuren, Arko Gorter et al. Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer International Journal of Oncology 6/1/2017 [PMID: 28498437]
• MJ Carroll, A Kapur, M Felder, MS Patankar, PK Kreeger M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop Oncotarget, 2016-12-27;7(52):86608-86620. 2016-12-27 [PMID: 27888810] (Neutralization, Human)
• Kouichi Sakamoto, Ngin Cin Khai, Yuqing Wang, Rie Irie, Hideo Takamatsu, Hiroshi Matsufuji et al. Heparin-binding epidermal growth factor-like growth factor and hepatocyte growth factor inhibit cholestatic liver injury in mice through different mechanisms International Journal of Molecular Medicine 12/1/2016 [PMID: 27779646]
• Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells. PLoS ONE, 2016-01-22;11(1):e0147312. 2016-01-22 [PMID: 26800359] (Neutralization, Human)
• Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N MicroRNA-132 enhances transition from inflammation to proliferation during wound healing. J Clin Invest, 2015-06-29;125(8):3008-26. 2015-06-29 [PMID: 26121747] (Western Blot, Human)
• Yu V, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang Y, Lymperi S, Ferraro F, Raaijmakers M, Wu J, Zhou L, Rajagopal J, Kronenberg H, Baron R, Scadden D Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. J Exp Med, 2015-04-27;212(5):759-74. 2015-04-27 [PMID: 25918341] (IHC-P, Human)
• Suzukawa , Keigo, Tomlin , Julia, Pak , Kwang, Chavez , Eduardo, Kurabi , Arwa, Baird , Andrew, Wasserman , Stephen, Ryan , Allen F A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation. PLoS ONE, 2014-07-17;9(7):e102739. 2014-07-17 [PMID: 25033458] (Western Blot, Rat)
• Smith A, Bowers S, Stratman A, Davis G Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. PLoS ONE, 2013-12-31;8(12):e85147. 2013-12-31 [PMID: 24391990] (Neutralization, Human)
• Li L, Chakraborty S, Yang C, Hatanpaa K, Cipher D, Puliyappadamba V, Rehman A, Jiwani A, Mickey B, Madden C, Raisanen J, Burma S, Saha D, Wang Z, Pingle S, Kesari S, Boothman D, Habib A An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII. Oncogene, 2013-09-30;33(33):4253-64. 2013-09-30 [PMID: 24077285] (Neutralization, Human)
• Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol, 2013-04-15;201(2):279-92. 2013-04-15 [PMID: 23589494] (Neutralization, Human)
• Vukovic , Jana, Borlikova , Gilyana, Ruitenberg , Marc J, Robinson , Gregory, Sullivan , Robert K, Walker , Tara L, Bartlett , Perry F Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering. J Neurosci, 2013-04-10;33(15):6603-13. 2013-04-10 [PMID: 23575857] (IHC-P, Mouse)
• Ray , K C, Moss , M E, Franklin , J L, Weaver , C J, Higginbotham , J, Song , Y, Revetta , F L, Blaine , S A, Bridges , L R, Guess , K E, Coffey , R J, Crawford , H C, Washington , M K, Means , A L Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia. Oncogene, 2013-02-04;33(7):823-31. 2013-02-04 [PMID: 23376846] (IHC, Human)
• Eric S. McCoy, Bonnie Taylor-Blake, Mark J. Zylka CGRP alpha -Expressing Sensory Neurons Respond to Stimuli that Evoke Sensations of Pain and Itch PLoS ONE 5/1/2012 [PMID: 22563493]
• Tsuyoshi Oguma, Koichiro Asano, Katsuyoshi Tomomatsu, Motohiro Kodama, Koichi Fukunaga, Tetsuya Shiomi et al. Induction of Mucin and MUC5AC Expression by the Protease Activity of Aspergillus fumigatus in Airway Epithelial Cells The Journal of Immunology 7/15/2011 [PMID: 21685325]
• Olivier Nolan-Stevaux, Morgan C. Truitt, Jessica C. Pahler, Peter Olson, Cristina Guinto, David C. Lee et al. Differential Contribution to Neuroendocrine Tumorigenesis of Parallel Egfr Signaling in Cancer Cells and Pericytes Genes & Cancer 10/27/2010 [PMID: 20975924]
• Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. J. Invest. Dermatol., 2010-04-29;130(8):2031-40. 2010-04-29 [PMID: 20428186] (Neutralization, Human)
• Mulder GM, Nijboer WN, Seelen MA, Sandovici M, Bos EM, Melenhorst WB, Trzpis M, Kloosterhuis NJ, Visser L, Henning RH, Leuvenink HG, Ploeg RJ, Sunnarborg SW, van Goor H Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury. J. Pathol., 2010-06-01;221(2):183-92. 2010-06-01 [PMID: 20225242] (IHC-P, Human, Mouse, Rat)
• Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors. Clin. Cancer Res., 2009-07-21;15(15):4829-37. 2009-07-21 [PMID: 19622585] (IHC-P, Human)
• Monslow J, Sato N, Mack JA, Maytin EV Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR. J. Invest. Dermatol., 2009-02-19;129(8):2046-58. 2009-02-19 [PMID: 19225541] (Western Blot, Human, Rat)
• Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Eur. Respir. J., 2007-09-05;30(6):1064-73. 2007-09-05 [PMID: 17804444] (Neutralization, Human)
• Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 2007-06-28;37(5):578-88. 2007-06-28 [PMID: 17600311] (Neutralization, Human)
• Casas-Gonzalez P, Garcia-Sainz JA Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation. Eur. J. Pharmacol., 2006-05-27;542(1):31-6. 2006-05-27 [PMID: 16828079] (Neutralization, Rat)
• Rubio I, Rennert K, Wittig U, Beer K, Durst M, Stang SL, Stone J, Wetzker R Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells. Biochem. J., 2006-09-01;398(2):243-56. 2006-09-01 [PMID: 16709153] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Li Y, Fan J, Chen M, Li W, Woodley DT Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. J. Invest. Dermatol., 2006-05-11;126(9):2096-105. 2006-05-11 [PMID: 16691197] (Neutralization, Human)
• Sun XX, Gemzell-Danielsson K, Li HZ, Stabi B, Stavreus-Evers A Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone. Fertil. Steril., 2006-01-01;85(1):171-8. 2006-01-01 [PMID: 16412750] (IHC, Human)
• Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Development, 2006-01-11;133(4):751-9. 2006-01-11 [PMID: 16407398] (ICC, Neutralization, Human)
• Kim J, Adam RM, Freeman MR Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress. Cancer Res., 2005-09-15;65(18):8242-9. 2005-09-15 [PMID: 16166300] (ICC, Human)
• Kansra S, Johnson JL Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Mol. Biol. Cell, 2004-07-14;15(9):4299-309. 2004-07-14 [PMID: 15254267] (Neutralization, Human)
• Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Dev. Biol., 2004-02-15;266(2):223-37. 2004-02-15 [PMID: 14738873] (ICC, Neutralization, Human)

Bioinformatics

• Gene Symbol: HBEGF
• Uniprot:
  • Q99075()