HARS Antibody (SR1884) - BSA Free

Western Blot: HARS Antibody (SR1884) [NBP3-22515] - Western blot analysis using NBP3-22515 in 293T cell lysate.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: SR1884
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HARS Antibody (SR1884) - BSA Free Summary

• Description: Novus Biologicals Rabbit HARS Antibody (SR1884) - BSA Free (NBP3-22515) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human HARS (Uniprot #: P12081)
• Specificity: Detects endogenous levels of total HARS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HARS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HARS Antibody (SR1884) - BSA Free

• cytoplasmic
• EC 6.1.1.21
• FLJ20491
• histidine-tRNA ligase
• histidyl-tRNA synthetase
• histidyl-tRNA synthetase, cytoplasmic

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HARS1