Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.

Flow Cytometry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401]

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Western blot analysis using GUCY1A3 mAb against HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.

ELISA: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 3G6B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free Summary

• Description: Novus Biologicals Mouse Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free (NBP2-37401) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Guanylyl Cyclase alpha 1 (AA: 22-214) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GUCY1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 77.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

• EC 4.6.1.2
• GC-S-alpha-1
• GCS-alpha-1
• GCS-alpha-3
• guanylate cyclase 1, soluble, alpha 3
• guanylate cyclase soluble subunit alpha-3
• Guanylyl Cyclase alpha 1
• GUC1A3
• GUC1A3GUCA3
• GUCSA3
• GUCSA3GC-SA3
• GUCY1A1
• GUCY1A3
• Soluble guanylate cyclase large subunit

Background

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GUCY1A1