NOSIP Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NOSIP Antibody [NBP1-85661] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Staining of human lung shows strong cytoplasmic positivity in macrophages.

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Staining of human cervix shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.

Analysis using Anti-NOSIP antibody NBP1-85661 (A) shows similar pattern to independent antibody NBP2-55693 (B).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOSIP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NOSIP Antibody - BSA Free (NBP1-85661) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGT
• Predicted Species: Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOSIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NOSIP Protein (NBP1-85661PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NOSIP Antibody - BSA Free

• CGI-25
• eNOS interacting protein
• eNOS-interacting protein
• nitric oxide synthase interacting protein
• nitric oxide synthase-interacting protein

Background

Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOSIP